Determination of trolline in flowers of Trollius chinensis by HPLC / 中国中药杂志

An HPLC method was developed for determinantion of trolline in the flowers of Trollius chinensis commercially available in China. The HPLC analysis was conducted on an Agilent C18 ODS column (4.6 mm x 250 mm, 5 microm) with acetonitrile-0.5% acetic acid solution (gradient: 0-30 min, 2:98-100:0) as mobile phase at a flow rate of 1.0 mL x min(-1). The detecting wavelength was at 258 nm. Linearity of trolline was good in the range of 0.1380-1.2410 microg and the content of trolline in nine batches of the flowers of T. chinensis commercially available in China was ranged from 0.05% to 0.11%. This method is good in terms of precision, accuracy and repeatability and can be used for the quantitative determination of trolline.