ABSTRACT
After a description of the emergency and development of subject librarian system, the subject librarian system in universities of USA and China was comparatively analyzed in aspects ofits management mechanisms , litera-cy of subject librarians and their duties.The problems in relation to subject service in domestic academic libraries were pointed out with suggestions put forward for their solution in order to further perfect the subject librarian system in domestic academic libraries.
ABSTRACT
Objective To explore the existance of ZASP(Z-band alternatively spliced PDZ-motif protein)gene mutations in idiopathic dilated cardiomyopathy(IDCM)patients in Chengdu and to study the relationship between this gene and IDCM.Methods Polymerase chain reaction-single-strand conformation polymorphism(SSCP)and DNA sequencing techniques were used to screening the possible mutation site of the ZASP gene exon 4,6,10,in the unrelated Han ethnic population of Chengdu area(including 120 IDCM patients and 100 normal controls).Results The difference of SSCP patterns were found on exon 10 of ZASP gene between IDCM and control groups.The DNA direct sequencing analysis of exon 10 revealed heterozygote G216T and homozygote T216T.G216T was only founded in 28 IDCM patients and 12 controls.T216T was only found 9 patients and 4 controls.Compared with controls,IDCM patients had different frequencies of the GT genotype and T allele(P