ABSTRACT
Objective To observe the effect of dihydromyricetin of ampelopsis grossedentata on lipid metabolism and antioxidation in atherosclerosis (AS) rats. Methods Sixty Wistar rats were randomly divided into five groups (n = 12 each):normal control group, model control group, positive control group, high dose dihydromyricetin group (40 mg·kg-1 ) and low dose dihydromyricetin group (10 mg·kg-1 ). Except normal control group, rats in the other groups were injected with a single dose of vitamin D3(600 000 U·kg-1 ) and loaded with high fat diet to establish AS model. Simvastatin (5 mg·kg-1 ) was intragastrically administered to positive control group. High and low dose dihydromyricetin groups received intragastric administration of 40 and 10 mg·kg-1 dihydromyricetin, respectively. Equal volume of purified water was given to normal and model group. After 24 weeks of administration, serum levels of lipids, activities of superoxide-dismutase (SOD) and malonicdialdehyde (MAD) were determined. Results As compared with model control group, triglycerides (TG) of high and low dose dihydromyricetin groups was decreased [(191. 65±101. 10) vs. (111. 10±29. 29) and (120. 55±38. 12) mg·L-1 , respectively], total cholesterol (TC) was decreased [(151. 64±33. 62) vs. (148. 49±30. 14) and (118. 90±27. 38) mg·L-1 ], and high density lipoprotein cholesterol (HDL-C) was increased [(1. 29±0. 68) vs. (2. 10±0. 70) and (1. 62±0. 61) mmol·L-1 ], low density lipoprotein cholesterol (LDL-C) was decreased [(5. 01±1. 33) vs. (3. 97±0. 78) and (4. 28±0. 79) mmol·L-1 ], activity of SOD was increased [(141. 03±42. 52) vs. (187. 97±42. 08) and (150. 99±46. 17) U·mL-1 ], and MDA was decreased [(20. 51±3. 81) vs. (17. 64±1. 54) and (18. 52± 3. 42) nmol·mL-1 ], with significant differences (P<0. 05, P<0. 01). Conclusion Dihydromyricetin can reduce the level of serum lipid, improve antioxidation activity, and has therapeutic effect for atherosclerosis.