ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular biological mechanism of arnebia root oil in promoting wound surface healing by observing histological change and basic fibroblast growth factor(bFGF) mRNA expression in the wound surface tissues of 2 groups, as well as the wound surface healing rate.</p><p><b>METHOD</b>Experimental model of incised-wound was produced on the back of 18 New Zealand albino rabbits. The wound surfaces were randomly divided into two groups, namely, experimental group and control group. The wound surfaces in the experimental group were treated by arnebia root oil and those in control group were treated by petrolatum gauze. Then raw surfaces were evaluated by the techniques of histology, histochemistry and electron microscope and the healing rates of the raw surfaces were compared between the two groups. Content of bFGF and it's mRNA expression in wound surface tissue was also measured by means of Western-blot and RT-PCR.</p><p><b>RESULT</b>The wound surface healing rate in experimental group was higher than that in control group( P < 0.05). The fibroblast, collagen and blood capillaries were comparatively richer in experimental group as compared with those in control group, and similarly, the expression of bFGF mRNA was also significantly enhanced in the experimental group as compared with control group during the various periods of treatment. In addition, the changes in the expressions of bFGF and it's mRNA paralleled the changes of healing rates in the two groups.</p><p><b>CONCLUSION</b>the present results showed that amebia root oil significantly can promote the healing of raw surfaces, which may be mediated by up-regulation of bFGF expression.</p>
Subject(s)
Animals , Female , Male , Rabbits , Boraginaceae , Chemistry , Fibroblast Growth Factor 2 , Genetics , Plant Oils , Pharmacology , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , RNA, Messenger , Genetics , Random Allocation , Skin , Wounds and Injuries , Metabolism , Pathology , Wound Healing , Wounds and Injuries , Metabolism , PathologyABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of comprehensive treatment for hip fracture in old people.</p><p><b>METHODS</b>Three hundred and seventy-two old patients with hip fractures were randomly divided into two groups, Group A and Group B. Cases in Group A were treated only by operations. Cases in Group B received comprehensive treatment. The Singh Indexes of both uninjured and injured femoral necks were used to judge the osteoporosis levels before operation and one year after the operation. The function of injured hip joints was evaluated one year postoperatively.</p><p><b>RESULTS</b>Complications occurred in 36.56% of the cases in Group A and 5.91% of Group B. One year postoperatively, the Singh Index degree distributions of both uninjured and injured femoral necks in Group A had no significant difference compared with those before the operation (P>0.05). In Group B, there was significant difference between one year postoperatively and before operation, and the Singh Index one year after the operation showed better result than that before operation (P<0.05). One year after operation, there was significant difference in the function of injured hip joints between Group A and Group B (P<0.05).</p><p><b>CONCLUSIONS</b>Hip fracture in old people should be treated comprehensively according to its internal characteristics, osteoporosis.</p>
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Chi-Square Distribution , Combined Modality Therapy , Comprehensive Health Care , Hip Fractures , Therapeutics , Osteoporosis , Postoperative Complications , Treatment OutcomeABSTRACT
Objective To evaluate the clinical results of free vascularized fibular associated with iliac graft in treatment of old femoral neck fracture.Methods From 1994 to 1997,76 cases of old femoral neck fracture were treated with free vascularized fibular associated with iliac graft,they were followed up for observ- ing fracture healing,hip function and femoral head necrosis.Results Sixty-eight of these 76 cases were fol- lowed up with detail data with the main term as 10.5 years.All fractures healed within 4 to 6 months.63 cases got a almost normal hip function with the Harris score was 87.5,in these cases,the structure of femoral head were normal and the grafted fibular and iliac bone healed with the femoral.4 cases got to femoral head necrosis and 1 experienced hip dislocation.Conclusion Free vascularixed fibular associated with iliac graft is a good method to treat these cases with old femoral neck fracture who want reserve their femoral head.