ABSTRACT
High-protein anti-D reagents prepared from pools of human serum have been used for routine RhD typing but, low-protein, saline reactive anti-D reagents formulated predominantly with monoclonal antibodies are in current use. Because some of the high-protein reagents contain macromolecular additives that may cause red cells coated with immunoglobulin to aggregate spontaneously, antisera with these additives may produce a false-positive reaction. A four-day old male was admitted due to severe jaundice. Initially, the RhD type of the newborn using a high-protein reagent was D-positive and then, using two low-protein reagents, it was D-negative. The blood type of the mother was B, CDe, and that of the newborn was B, CcdEe. The direct antiglobulin test on the newborn's RBC was positive. Anti-E and anti-c were identified in the mother's serum and anti-E only was identified in the newborn's serum. The newborn was treated with phototherapy for 10 days and discharged as recovered. We present a case of hemolytic disease of the D negative newborn, which showed a discrepancy between high protein anti-D and low protein anti-D. With a review of literature, the newborn was possibly misinterpreted as D positive.
Subject(s)
Humans , Infant, Newborn , Male , Antibodies, Monoclonal , Coombs Test , Immune Sera , Immunoglobulins , Indicators and Reagents , Jaundice , Mothers , PhototherapyABSTRACT
Idiopathic Myelofibrosis (IMF), a clonal disorder of a multipotent hemtopoietic progenitor cell of unknown etiology, is characterized by massive splenomegaly, leukoerythroblastic blood changes, and bone marrow fibrosis. Portal hypertension occurs in approximately 10% of patients with IMF. Alsom there mey be bleeding from esophageal varices. A 59-year-old woman was admitted with intermittent abdominal distension and melena. An esophagogastroduodenoscopy revealed extensive esophageal varices with red colored signs, and subsequently esophageal variceal ligation was performed. Marked splenomegaly and ascites was observed through abdominal US and MRI scan. Bone marrow biopsy revealed an increase in reticulin and fibrosis. Extramedullary hematopoiesis and sinusoidal change were observed in the liver biopsy specimen. This case of IMF causing an esophageal variceal hemorrhage is herein reported.
Subject(s)
Female , Humans , Middle Aged , Ascites , Biopsy , Bone Marrow , Endoscopy, Digestive System , Esophageal and Gastric Varices , Fibrosis , Hematopoiesis, Extramedullary , Hemorrhage , Hypertension, Portal , Ligation , Liver , Magnetic Resonance Imaging , Melena , Primary Myelofibrosis , Reticulin , Splenomegaly , Stem CellsABSTRACT
BACKGROUND: Since method for calculating the risk of Down's syndrome pregnancy using the mothers alphafetoprotein(AFP), chorionic gonadotropin(CG) and unconjugated Estriol(uE3) levels in serum was developed, many reports showed the method including triple markers was more effective than calculating the risk only by the mother's age. Authors developed a computer program which could calculate the risk conveniently. We compared it with the established program and explained the calculating logic used in the program to help the readers to make their own computer program. METHODS: The risk of Down's syndrome pregnancy for age was calculated by the method of Cuckle, and medians of the CG, AFP, uE3 according to the days of pregnancy was calculated by Wald. Delphi(version 4.5, Inprise/Borland Corp., Scotts Valley CA, USA) was used as a programing language. We compared the risks of the pregnant women previously reported by the established computer program with those calculated by new computer program. RESULTS: In this program, user put down patient's demographic information and the results of each tests and press the command button then the user can see the calculated risk in one screen. User can use newly calculated median value according to their own accumulated data. There were almost no differences in risks between the program and formerly established one. CONCLUSIONS: The computer program developed by authors was agreed well with the established one, so this program could replace the latter. We hope there are many programs developed by readers in the future.
Subject(s)
Female , Humans , Pregnancy , Chorion , Down Syndrome , Hope , Logic , Mass Screening , Mothers , Pregnant Women , Risk FactorsABSTRACT
BACKGROUND: A two-dose measles vaccination schedule is recommended routinely for either school entry or 11 to 13 years of age in America since 1989. But, several studies were performed on measles antibody in Korea and it remains controversial whether a second dose measles vaccine after 15 months is necessary. To generate baseline data, measles antibody prevalence and its levels according to different age groups in children and young adults in Taejon area were studied. METHODS: A total of 261 subjects at 3 to 21 years of age, who had received a single dose of measles vaccine, were tested for measles antibody by quantitative alpha enzyme immunoassay. The subjects were divided into five age-groups based on the educational system (preschool, elementary school, middle school, high school, young-adult). RESULTS: The seropositivity rates were 97.7% and not significantly different among groups. The expected tendency of declining antibody levels with advancing age, as reported by other studies, was not observed in this study. Except for between Group I and Group II, no significant difference was noted in the antibody levels among the five age groups. Group II showed significantly higher antibody levels than those of Group I (P=0.0025). CONCLUSIONS: No declining tendency of measles antibody levels with advancing age is different from many other studies and contradicts the current recommendations for supplementary vaccinations after 15 months. These might reflect the regional characteristics of the study population in Taejon area and current vaccination rate. Therefore, futher studies with larger population in different geographic regions by quantitative EIA would be needed.
Subject(s)
Child , Humans , Young Adult , Americas , Appointments and Schedules , Immunoenzyme Techniques , Korea , Measles Vaccine , Measles , Prevalence , VaccinationABSTRACT
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Subject(s)
Base Sequence , Chlorocebus aethiops , DNA , Encephalitis , Glycoproteins , Herpes Simplex , Herpesvirus 1, Human , Herpesvirus 2, Human , Keratitis , Kidney , Mouth Mucosa , Mucous Membrane , Polymerase Chain Reaction , Sensitivity and Specificity , Sexually Transmitted Diseases , Simplexvirus , Skin , Uterine Cervical NeoplasmsABSTRACT
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Subject(s)
Base Sequence , Chlorocebus aethiops , DNA , Encephalitis , Glycoproteins , Herpes Simplex , Herpesvirus 1, Human , Herpesvirus 2, Human , Keratitis , Kidney , Mouth Mucosa , Mucous Membrane , Polymerase Chain Reaction , Sensitivity and Specificity , Sexually Transmitted Diseases , Simplexvirus , Skin , Uterine Cervical NeoplasmsABSTRACT
No abstract available.