ABSTRACT
Objective The study discusses the clinical efficacy, surgical technique and new technology application of "⊥" incision in tracheotomy. Methods From March 2014 to December 2015, a total of 300 patients treated in the First Affiliated Hospital of Kunming Medical University with tracheotomy prospective were studied.The statistics of success rate of incision, balloon cuff rupture and complications of "⊥" form incision (groupA) and"I"form incision (group B) during tracheotomy.Result In group A, 144 out of 150 cases (96%) were inserted successfully for the first try,while the first incision was successful in 90/150 cases of group B (60%) (P＜0.01) . Balloon cuff rupture was found in 3/150 cases (2%) in groupA and 15/150 cases (10%) in group B (P＜0.05) . The postoperative complications (postoperative bleeding, subcutaneous emphysema, pneumothorax,laryngotracheal stenosis) were observed in 10/150 cases (6.7%) in groupA and 25/150 cases (16.7%) in group B (P＜0.05).Conclusion The"⊥" tracheal incision is simple, safe and causes fewer complications. It is worthy of generalization and application.
ABSTRACT
Objective To investigate the effects of anticardiolipin antibodies positive serum from patients with recurrent pregnancy loss on the proliferation of BeWo cells as well as the modulation of heparin on the growth of BeWo cell.Methods Thirty patients with recurrent pregnancy loss whose anticardiolipin antibodies were positive and thirty healthy women with a history of term delivery were selected.Their sera were separately added to BeWo cell culture systems which contained either heparin or not.After 24 hours, the PCNA and 490 nm A value were detected by immunofluorescence and methyl thiazolyl tetrazolium (MTT),and the proliferation of BeWo cell was evaluated.Results In the culture systems with heparin and normal serum,normal serum,heparin and serum with positive autoantibody,serum with positive autoantibody,the mean fluorescence gray scale values of PCNA were 34.8?3.1,33.8?1.8,33.4?2.2 and 25.1?2.3,the 490 nm A values were 0.0560?0.0033,0.0535?0.0024,0.0524?0.0027 and 0.0350?0.0040,respectively.Conclusions The serum with anticardiolipin antibodies from patients with recurrent pregnancy loss can influence the prospective potency of BeWo cell,inhibit cell proliferation in vitro.Through this direct effect on biological behaviour of the trophoblastic cell,it will lead to recurrent pregnancy loss,whereas heparin can increase the ratio of pregnancy by reversing such effects in those patients.