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Objective To evaluate the clinical efficacy and safety of dendritic cell (DC) combined with cytokine induced killer cell (CIK) in treatment of advanced non-small-cell lung carcinoma (NSCLC).Methods Peripheral blood mononuclear cells (PBMCs) were collected from 39 patients with advanced NSCLC,who were admitted to Affiliated Hospital of Academy of Military Medical Sciences,and cultured in vitro to produce DCs and CIK cells which,after phenotypic characterization by flow cytometry,were then returned to the patients.DCs were given subcutaneously on day 7,9,11 and 13 and CIK cells were given intravenously on day 11 and 13.The clinical efficacy and safety were analyzed before and after DCs-CIK cells treatment.Results Following up displayed that,in 39 patients with advanced NSCLC and eligible for evaluation,the objective response rate (ORR) was 30.8％ including 2 cases of completed response (CR) and 10 cases of partial response (PR),the disease control rate (DCR) was 69.2％.No significant difference existed pre-and post-treatment on the proportion of T cell subsets including CD3+CD4+CD8ˉ,CD3+CD4ˉ CD8+,CD3+CD19ˉ,CD3ˉCD 19+,CD3 CD16+CD56+,CD3+CD16+D56+,CD3+HLA-DRˉ,CD3+HLA-DR+ and CD3+CD28+CD8+ (P＞0.05),while obvious changes were found in Th1,Th2 and CD3+CD4+CD25+ T cells (Treg cells) (P＜0.05).No serious adverse events were observed.Conclusion Combined DCs-CIK cells immunotherapy provides a safe and effective treatment for patients with advanced NSCLC,improves the quality of life and relieves the probability of metastasis and recurrence.
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Objective To evaluate the clinical efficacy, the immune function and follow-up observation of autologous dendritic cells (DCs) combined with cytokine-induced killer (CIK) cells in the treatment of metastatic renal cell carcinoma. Methods Peripheral blood mononuclear cells (PBMCs) were collected from 27 patients with metastatic renal cell carcinoma, and cultured in vitro to produce DCs and CIK cells. After sterility test, phenotypic character ization by flow cytometry and cell count, the produced DCs and CIK cells were then returned to the patient. DCs were given subcutaneously on day 7, 9, 11 and 13 respectively, after PBMCs collection, and CIK cells were given intravenously on day 11 and 13 respectively. This treatment regimen was repeated at a 3 months interval until the disease progresses. Clinical outcomes and immune function were recorded during the treatment period. Results After DCs-CIK cells treatment, clinical efficacy showed an objective response rate (ORR) of 37%, a disease control rate (DCR) of 85% and 2 years overall survival rate of 81.5%. There were no significant changes of T cell subsets including CD3+CD4+CD8–, CD3+CD4–CD8+, CD3+CD19–, CD3–CD19+, CD3–CD16+CD56+, CD3+CD16+CD56+, CD3+HLA-DR–, CD3+HLA-DR+, CD3+CD28+CD8+ and Th2 cells except CD3+CD4+CD25+ T cells (Treg cells) and Th1 in peripheral blood between pre- and post-treatment. No serious adverse events were observed. Conclusion DCs-CIK cells immunotherapy provides a safe and effective treatment approach for patients with metastatic renal cell carcinoma, and may improve the immunosuppression status and enhance the anti-tumor immunity without obvious adverse reaction.
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<p><b>BACKGROUND</b>Glucagon-like peptide-1 (GLP-1) reduces fatty acid-induced beta-cell lipotoxicity in diabetes; however, the explicit mechanisms underlying this process are not fully understood. This study was designed to investigate the involvement of microRNA, which regulates gene expression by the sequence-specific inhibition of mRNA transcription in the GLP-1 mediation of beta-cell function.</p><p><b>METHODS</b>The cell viability and apoptosis were determined using an methyl thiazoleterazolium (MTT) assay and flow cytometry. The expression of genes involved in beta-cell function, including microRNA-34a and sirtuin 1, were investigated using real-time PCR. The underlying mechanisms of microRNA-34a were further explored using cell-transfection assays.</p><p><b>RESULTS</b>A 24-hours incubation of INS-1 cells with palmitate significantly decreased cell viability, increased cell apoptosis and led to the activation of microRNA-34a and the suppression of sirtuin 1. A co-incubation with GLP-1 protected the cells against palmitate-induced toxicity in association with a reduction in palmitate-induced activation of microRNA-34a. Furthermore, palmitate-induced apoptosis was significantly increased in cells that were infected with microRNA-34a mimics and decreased in cells that were infected with microRNA-34a inhibitors.</p><p><b>CONCLUSION</b>MicroRNA-34a is involved in the mechanism of GLP-1 on the modulation of beta-cell growth and survival.</p>
Subject(s)
Animals , Rats , Apoptosis , Cell Line , Cell Survival , Fatty Acids, Nonesterified , Toxicity , Glucagon-Like Peptide 1 , Pharmacology , Insulin-Secreting Cells , Cell Biology , Metabolism , MicroRNAs , Genetics , Metabolism , Palmitic Acid , Pharmacology , Real-Time Polymerase Chain ReactionABSTRACT
<p><b>OBJECTIVE</b>To study the antioxidative and antitumor activities of flavonoids isolated from Epimedium koreanum.</p><p><b>METHOD</b>The compounds were separated by column chromatography with silica gel and Sephadex LH-20, and identified by spectral a- nalysis (ESI-MS, 1H-NMR and 13C-NMR) respectively. DPPH radical scavenging assay and MTT assay were used to observe the antioxidative and antitumor abilities.</p><p><b>RESULT</b>Six compounds were isolated from the the ethyl acetate extract of the aerial part. Their structures were identified as icariin (I), luteolin (II), baohuoside II (III), hyperoside (IV), epimedokoreanin B (V) and baohuoside I (VI). The results indicated that at concentrations of 3. 125-200 micromol x L(-1), compound I, III and VI had no ability to scavenge the DPPH radical, but the scavenging ability of compounds II, IV and V were stronger than that of Vit C in dose-dependant manner. Compounds I, II, V and VI could inhibit the proliferation of MCF-7 and HepG2 in dose-dependant manner, but compounds III and IV had no effect on the proliferation.</p><p><b>CONCLUSION</b>The antitumor activity of E. koreanum may be partially related to the antioxidantive activity of flavonoids.</p>
Subject(s)
Female , Humans , Antineoplastic Agents, Phytogenic , Pharmacology , Antioxidants , Pharmacology , Breast Neoplasms , Pathology , Cell Line, Tumor , Cell Proliferation , Dose-Response Relationship, Drug , Epimedium , Chemistry , Flavonoids , Pharmacology , Liver Neoplasms , Pathology , Luteolin , Pharmacology , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of the rhizomes of Actaea asiatica in order to obtain a more comprehensive understanding of its effective components.</p><p><b>METHOD</b>Compounds were separated by silica gel chromatography, RP-C18 chromatography and semi-preparative high performance liquid chromatography, and their structures were established by spectral analysis and chemical evidence.</p><p><b>RESULT</b>Six compounds were isolated from the ethyl acetate extract. Their structures were identified as 25-O-acetylcimigenol (1), 12beta-hydroxycimigenol (2), 23-epi-26-deoxyactein (3), 27-deoxyacetylacteol (4), 26-deoxycimicifugenin (5) and beta-sitosterol (6).</p><p><b>CONCLUSION</b>All these compounds mentioned above were isolated from the plant for the first time.</p>
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Actaea , Chemistry , Lanosterol , Chemistry , Plants, Medicinal , Chemistry , Rhizome , Chemistry , Saponins , Chemistry , Sitosterols , Chemistry , Triterpenes , ChemistryABSTRACT
To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.
Subject(s)
Base Sequence , Biodiversity , China , Cluster Analysis , DNA, Plant , Chemistry , Genetics , DNA, Ribosomal , Chemistry , Genetics , DNA, Ribosomal Spacer , Chemistry , Genetics , Genotype , Geography , Lamiaceae , Classification , Genetics , Molecular Sequence Data , Phylogeny , Plant Leaves , Genetics , Plants, Medicinal , Classification , Genetics , Sequence Analysis, DNAABSTRACT
The anti-osteoporosis activity and mechanism of traditional Chinese medicine and medicinal plants were discussed. It is hoped that we can provide some reference for future drug development and introduction of traditional Chinese medicine to world.
Subject(s)
Animals , Humans , Cell Proliferation , Cnidium , Chemistry , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Epimedium , Chemistry , Medicine, Chinese Traditional , Osteoblasts , Pathology , Osteoporosis , Pathology , Phytotherapy , Plants, Medicinal , Chemistry , Polypodiaceae , ChemistryABSTRACT
The influences of E23K polymorphism of inwardly rectifying K~+channel 6.2 (Kir6.2) gene on the clinical phenotype of type 2 diabetes and glucose-lowering effect of gliclazide were investigated.The result showed that E23K polymorphism did not influence glucose-lowering effect of gliclazide,but serum creatinine level of patients with K/K genotype was higher than those of E/E and E/K genotypes (P