ABSTRACT
<p><b>OBJECTIVE</b>To study the adsorption and enrichment of effective compounds of danshensu, and protocatechualdehyde from Danshen water extracts by macroporous resin D301.</p><p><b>METHOD</b>The change of active compounds was observed by detecting the amount of danshensu and protocatechualdehyde with HPLC.</p><p><b>RESULT AND CONCLUSION</b>Macroporous resins D301 can be used in the process of adsorbing and purifying active compounds of danshensu and protocatechualdehyde, and increase the amount of active compounds in water extracts.</p>
Subject(s)
Adsorption , Anion Exchange Resins , Benzaldehydes , Catechols , Chromatography, High Pressure Liquid , Lactates , Plants, Medicinal , Chemistry , Salvia miltiorrhiza , Chemistry , Technology, Pharmaceutical , MethodsABSTRACT
The antifungal antibiotic produced by Streptomyces luteogriseus H-103 was purified by means of macroporous adsorbent resin, and the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of microporous adsorbent resin and detection by reversed high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) were established. The result appeared that resin X-5 is the best adsorbent, the eluant is 50% ethanol. The antibiotic was successfully separated on Agilent~(TM)20RBA?310SB C_(18 )column (150mm?4.6mm i.d,5?m) , using a mixture of acetonitrile (A)-H_(2)O (B) as a mobile phase under gradient elution at a flow of 0.8mL/min at 30℃.0~4.0 min, V(A)∶V(B)=20∶80, 4.0~9.5min, V(A)∶V(B)=45∶55, then V(A)∶V(B)=80∶20. The drift tube temperature and the air carrier gas flow rate of the ELSD were set at 115℃ and 2.3L/min.