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OBJECTIVE@#To investigate the protective effects of Shexiang Tongxin Dropping Pill (, STDP) following sodium laurate-induced coronary microembolization (CME) in rats.@*METHODS@#Forty rats were divided into 4 groups: the control (sham) group, CME group, low-dose STDP pretreatment group (20 mg·kg@*RESULTS@#The rats in the CME group showed a significant increase in the fibrinogen-like protein 2 expression level and mitochondrial dysfunction and a decrease in the expression level of antioxidant biomarkers (superoxide dismutase and catalase, P<0.01 for all). In contrast, the rats in the low- and high-dose STDP pretreatment groups showed a significant decrease in coronary microthrombi (P<0.05); moreover, STDP restored the antioxidant-related protein activities and mitochondrial function, inhibited mPTP opening, decreased AKT-Ser473 phosphorylation, and increased GSK3β-Ser9 phosphorylation (P<0.05 or P<0.01).@*CONCLUSION@#STDP may be useful for treatment of CME, possibly via regulation of mPTP opening and AKT/GSK3β phosphorylation.
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Objective To evaluate the effect of OTD (observation-teaching-discussion) teaching for undergraduate students major-ing in rehabilitation therapy. Methods Students in Grade 2013 and Grade 2014 for rehabilitation therapy were taught with traditional teaching and OTD teaching in course of physiotherapy. The scores of theory and practice, and total score of the final test of physiotherapy were compared, and their satisfaction for the course was investigated with questionnaire. Results The scores of theory and practice, and total score were all greater in the Grade 2014 than in the Grade 2013 (t>2.73, P<0.01), as well as the satisfaction for the course (t=3.17, P=0.005). Conclusion OTD teaching may improve the achievement of study and satisfaction for the course.
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Objective To explore the influence of long -term smoking on the level of blood inflammatory cytokines and the degree of atherosclerosis in healthy middle -aged men.Methods A total of 84 middle -aged men who accepted the health examination were divided into smoking group and non -smoking group.The risk factors of atherosclerosis,such as age,body mass index (BMI),systolic blood pressure (SBP),diastolic blood pressure (DBP),fasting blood glucose (GLU),total cholesterol (TC),low density lipoprotein (LDL),high density lipoprotein (HDL)and triglyceride (TG) were recorded.The white blood cell (WBC)level and neutrophil cell (NC)level were also recorded.The interleukin -6 (IL -6)concentration was assayed by using enzyme -linked immunosorbent double antibody method and the high -sensitivity C reactive protein (Hs -CRP)concentration was assayed by using immune turbidimetry method.The carotid artery intima -media thickness (IMT)and plaques were measured by B -mode ultrasonography.Results There were no significant differences in the risk factors of atherosclerosis between the two groups.But there were statistically significant differences between smoking group and non -smoking group in IL -6 (1 .46 ±0.27 vs.1 .21 ±0.24),Hs -CRP (6.57 ±2.47 vs.3.1 4 ±2.07),WBC (5.78 ±0.79 vs.5.25 ±0.64)and NC (3.40 ±0.45 vs.2.87 ±0.42)(P <0.05). The carotid artery IMT in smoking group was significantly thicker than that of the non -smoking group (1 .0 ±0.1 8 vs. 0.82 ±0.1 6,P <0.01 ).The detection rate of plaque and carotid stenosis in smoking group was significantly higher than that of the non -smoking group (P <0.01 ).Conclusion Long -term smoking not only leads to increased inflammatory cytokines,but also aggravates the degree of atherosclerosis in healthy middle -aged men.Long -term smoking healthy middle -aged men are potential in patients with cardiovascular disease.
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Typhonii Rhizoma, the dried tuber of Typhonium giganteum, is a kind of commonly used Chinese materia medica for the treatment of cancer, stroke, trigeminal neuralgia, facial paralysis, and many other illnesses, but its active and toxic components as well as molecular mechanisms have not been specified. In this paper, the herbal textual research, historical evolutions in Pharmacopoeia, chemical composition, pharmacology and toxicology, processing and quality control of Typhonii Rhizoma were summarized in detail, so as to provide the references for the rational development and utilization of Typhonii Rhizoma.
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<p><b>OBJECTIVE</b>To evaluate the analgesic effect of Corydalis yanhusuo on trigeminal neuropathic pain.in a rat model.</p><p><b>METHOD</b>Rat model of trigeminal neuralgia pain were established by inducing chronic constriction injury (CCI) of the infraorbital branch of the trigeminal nerve (ION). The effect of Corydalis yanhusuo, a traditional Chinese medicine, in ameliorating the pain was tested. Western blotting was performed to investigate the change of cannabinoid CB1 receptors in the Vc the injury of the infraorbital branch of the trigeminal nerve (ION-CCI). CB1 receptor antagonist AM 251 was applied to observe its effect on the analgesic effect of Yanhusuo.</p><p><b>RESULT</b>Administration of dl-THP (2 mg/kg) intraperitoneally increased the response threshold and the cut-off threshold to the mechanical stimulation in ION-CCI rat models. ION-CCI induced an upregulation of cannabinoid CB1 receptors within the ipsilateral of Vc. The effect of Yanhusuo was antagonized by the application of AM 251.</p><p><b>CONCLUSION</b>The analgesic effect of Yanhusuo involves the participation of CB1 receptors, suggesting that Yanhusuo may offer a useful therapeutic approach for trigeminal neuropathic pain.</p>
Subject(s)
Animals , Male , Rats , Analgesics, Non-Narcotic , Therapeutic Uses , Corydalis , Chemistry , Drugs, Chinese Herbal , Therapeutic Uses , Phytotherapy , Rats, Sprague-Dawley , Receptor, Cannabinoid, CB1 , Trigeminal Nerve Diseases , Drug TherapyABSTRACT
<p><b>OBJECTIVE</b>To investigate the mRNA and protein expressions of 11beta-Hydroxysteroid dehydrogenase type 2 (11betaHSD2) in patients with atrial fibrillation.</p><p><b>METHODS</b>Right and left atrial lateral wall tissue samples were obtained during mitral/aortic valve replacement operation from 25 patients with rheumatic heart valve disease (12 in sinus rhythm and 13 in chronic atrial fibrillation). Realtime quantitative PCR and Western blot were used to determine the mRNA and protein expressions of 11betaHSD2 in atria specimens. The distribution of 11betaHSD2 in human atrial tissue was analyzed by specific immunohistochemical staining. Echocardiography examination was performed before operation.</p><p><b>RESULTS</b>The left atrial diameters were significantly higher in the atrial fibrillation group as compared to sinus rhythm group (P < 0.01). Similarly, mRNA expression of 11betaHSD2 (0.86 +/- 0.14 vs 0.33 +/- 0.12 in right atria, 0.95 +/- 0.15 vs 0.37 +/- 0.10 in left atria, all P < 0.01) and protein expression of 11betaHSD2 (1.18 +/- 0.64 vs 0.71 +/- 0.21 in right atria, P < 0.01; and 1.36 +/- 0.58 vs 0.85 +/- 0.15 in left atria, P < 0.05) were also significantly upregulated in atrial fibrillation groups than those in sinus rhythm groups. The mRNA and protein expressions of 11betaHSD2 were similar between left atria and right atria both in fibrillation and sinus groups (all P > 0.05). The special immunohistochemical staining demonstrated that 11betaHSD2 was abundant in the human atrial myocardium and located mainly in the cytoplasm.</p><p><b>CONCLUSION</b>These findings suggested that upregulated 11betaHSD2 might be associated to the development and persistence of atrial fibrillation.</p>