ABSTRACT
To investigate the effect of selective N-methy-D-aspartate receptor antagonist MK-801 on dentate granule cell neurogenesis after diffuse brain injury in the adult rat, so as to explore the role of glutamic acid on dentate granule cell neurogenesis after diffuse brain injury. 45 adult male SD rats were subjected to diffuse brain injury and randomized into MK-801-treatment group, vehicle-treatment group and control group (n=15 each). By using bromodeoxyuridine ( BrdU ) labelling method and immunohistochemistry to observe dividing cells, the proliferation rates of neural precursor cells in the dentate gyrus(DG) were compared between MK-801-treatment group and corresponding control groups on 2, 4, 6, 8, and 12 days after diffuse brain injury. The results showed that MK-801 (1mg/kg i.p.) significantly reduced the number of BrdU labeled cells in the dentate gyrus on 4, 6, 8 and 12 days after diffuse brain injury (P
ABSTRACT
AIM To examine the effect of metabotropic glutamate receptor ligand (s) 4 carboxy 3 hydroxyphenyl glycine 〔(s) 4C3HPG〕 on kainic acid induced epileptic seizures and brain damage in rats. METHODS Rats were given (s) 4C3HPG (500 nmol/rat) by icv infusion prior to kainic acid (KA) injection ip(10 mg?kg -1 ). The behavoir changes, electrographic seizure duration and histological alterations between different group of rats were compared. RESULTS (s) 4C3HPG markedly reduced seizures severity in response to kainate. The seizure score in the rat with (s) 4C3HPG injection icv was significantly lower than that of control during the first three hours observation period after KA administration ( P
ABSTRACT
Objective To investigate the effect of electroacupuncture applied at Baihui acupoint on cognitive function in lithium-pilocarpine induced spontaneous recurrent epileptic rat and provide some theoretical basis for acupoint stimulation treatment seizure.Methods Male mature Sprague Dawley rats were subjected to spontaneous recurrent epileptic model induced by lithium-pilocarpine,and divided into different groups randomly: acupoint group(electroacupuncture applied at Baihui acupoint),non-acupoint group(electroacupuncture located in close vicinity to the Baihui acupoint),and epileptic control group.Normal rats were subjected into normal control group.The Morris water maze test and step though test were carried out respectively to explore the effect of electroacupuncture applied at Baihui acupoint on the rats' learning and memory capacities.Results Compared with control group,acupoint rats in Morris water maze test time of finding the platform under the water surface decreased(P