Mass Spectrometric Analysis of Proteoforms of Cyclophilin A from Human Colorectal Carcinoma Tissues / 分析化学

Cyclophilin A was isolated from human colorectal carcinoma tissues by ultrafiltration,affinity chromatography,and two dimensional electrophoresis.By the aid of Western blot analysis,several isoforms of cyclophilin A were detected.,The tryptic digests from cyclophilin A were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry as well as electrospray tandem mass spectrometry techniques.Several different modifications such as N-terminal acetylation (Ac-1Met and Ac-2Val) and phosphorylation of 5Thr were identified from these isoforms of cyclophilin A,which represented the majority of the proteoforms of cyclophilin A in this study.In addition,other kinds of modifications such as oxidation and deamidation were also found in these proteoforms.The results indicated that the developed method could be used to rapidly and correctly identify the several proteoforms of cyclophilin A isolated from human colorectal carcinoma tissues.

Relationship between cumulative noise exposure and hypertension among iron and steel production workers / 中国职业医学

OBJECTIVE: To analyze the relationship between the cumulative noise exposure( CNE) and prevalence of hypertension in iron and steel production workers. METHODS: By convenience sampling method,2 253 workers,whose workplace in an iron and steel enterprise with wet bulb globe temperature index less than 25. 0 ℃,were selected as study subjects. The blood pressure and workplace noise exposure level were measured. According to the equal energy principle,the normalized continuous A-weighted sound pressure level equivalent to an 8 h-working-day and workers' length of service were used to calculate the CNE level. The workers with CNE≥75 d B( A) ·year were regarded as the exposure group,and those with CNE < 75 d B( A) ·year were deemed as the control group. RESULTS: The prevalence rate of hypertension in the exposure group was higher than that of the control group( 32. 3% vs 21. 5%,P < 0. 01). With the increased CNE level,the prevalence rate of hypertension increased( P < 0. 01). The multiple logistic regression analysis results showed that the prevalence risk of hypertension disputed an upward trend of the dose-response relationship following the increasing CNE among the iron and steel production workers( P < 0. 01) after controlling the confounding factors of age,body mass index,educational background,family history of hypertension,smoking,alcohol drinking,salt intake,fried barbecue food intake and so on. CONCLUSION: CNE is a vital factor which influences hypertension prevalence in iron and steel production workers. Strengthen the noise protection is helpful for the prevention and treatment of hypertension in the steel workers.

Relationship between high-temperature working classification and hypertension among iron and steel production workers / 中国职业医学

OBJECTIVE: To analyze the relationship between the high-temperature working classification and prevalence of hypertension in iron and steel production workers. METHODS: By a convenience sampling method,2 798 workers in an iron and steel production enterprise were selected as study subjects. They were divided into high-temperature working group( 1 070 cases) and non-high-temperature working( control) group( 1 728 cases). The occupational health status was investigated by questionnaire survey and the blood pressure was measured. The wet bulb globe temperature in workplace of high temperature working group was measured,and the high-temperature classification was made based on the time of exposure to high temperature. RESULTS: The prevalence rate of hypertension of 2 798 iron and steel production workers was32. 8%. The prevalence rate of hypertension of high-temperature working group was higher than that of the control group( 41. 3% vs 27. 5%,P < 0. 01). The prevalence rate of hypertension in high temperature working group presented an increasing tendency with the increase of high-temperature working level( P < 0. 01). The multiple logistic regression analysis results showed that excluding the confounding factors of age,body mass index,family history of hypertension,educational background,smoking,alcohol drinking,salt intake and mental stress,it showed that high-temperature working classification was positively correlated with increasing prevalence of hypertension in high-temperature working group( P <0. 01); and the initial exposure age to high-temperature was negatively correlated with the prevalence of hypertension in iron and steel production workers( P < 0. 01). CONCLUSION: High-temperature is an important influence factor to the prevalence of hypertension in iron and steel production workers. Strengthening the management of high-temperature classification is helpful for the prevention and treatment control of hypertension in the iron and steel production workers