ABSTRACT
Objective:To explore the correlation between several single-nucleotide polymorphisms (SNPs) loci on the gene of human telomerase reverse transcriptase (hTERT)(including rs2736100、rs2853676) and the susceptibility to Hepatocellular carcinoma in Qingdao area.Methods:This case-control study included 165 diagnosed Hepatocellular carcinoma,195 with chronic hepatitis B and 402 Healthy people.Polymerase chain restriction fragment length polymorphism were performed to analyze the genotype of the loci on the gene of hTERT.Results:The frequency of TT genotype on rs2736100 were higher in the Hepatocellular carcinoma group compared to the control group、chronic hepatitis B and no HCC people(P＜0.05).The additive odds ratio of TT genotype for the risk of Hepatocellular carcinoma was 2.13、2.17 and 2.14.The frequency of AA genotype on rs2853676 were higher in the Hepatocellular carcinoma group compared to the control group、chronic hepatitis B and no HCC people (P＜0.05).The additive odds ratio ofTT genotype for the risk of Hepatocellular carcinoma was 3.42、2.39 and 3.02.Conclusion:There was a relationship between hTERT rs2736100 and rs2853676 polymorphism and susceptibility to Hepatocellular carcinoma.
ABSTRACT
<p><b>OBJECTIVE</b>To find out the connection of serum pepsinogen and it's subgroups (PGI, PGII) with CA72-4 to early diagnosis and postoperative recurrence on gastric cancer.</p><p><b>METHODS</b>RIA was applied to detect the results of serum PGI, PGII and CA72-4 on gastric cancer and other stomach diseases, then the clinic value of associating detection on gastric cancer diagnosis and prognosis judgment were assessed.</p><p><b>RESULTS</b>The serum PG levels of GC patients were significantly lower comparing to those of healthy controls (P < 0.01), apparent changes had taken place on earlier period GC (P < 0.05), and aggressive GC were even lower (P < 0.01). On the earlier period of GC diagnosis, CA72-4 levels were not apparently different to healthy controls (P > 0.05), and aggressive GC were significantly higher (P < 0.01). Compared preoperative with postoperative, the serum PGI and PGII and CA72-4 levels were significantly different (P < 0.01). In the patients underwent total gastrectomy, both of pepsinogen levels were lower than those of subtotal or large partial gastrectomy (P < 0.05). The serum PGI, PGII and CA72-4 levels of patients with recurrence of GC after total gastrectomy were significantly higher than those without. Compared before recurrence patients with after ones, the serum PGI and PGII levels of partial gastrectomy were no apparent difference (P > 0.05), however apparent changes had taken place on CA72-4 levels. The associate detection had even higher specificity (P < 0.01).</p><p><b>CONCLUSIONS</b>Apply to detect the serum PG levels on crowds, especially pGI, PGI/II levels decrease, which may be expected to become the index to earlier period GC screening. The associating detection to PG and CA72-4 levels may significantly improve sensitivity and specificity, which have chances to be applied to monitoring to postoperative gastrectomy.</p>