ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of intracytoplasmic sperm injection (ICSI) using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration (TEFNA) in patients with non-obstructive azoospermia.</p><p><b>METHODS</b>Sixty-two patients underwent TEFNA. Mature testicular spermatozoa were found in 35 cases of patients and the testicular tissues were cryopreserved for later ICSI. Ovarian stimulation included a long protocol of GnRHa/FSH/hCG. Oocyte retrieval was performed under transvaginal ultrasound guidance, and ICSI conducted with cryopreserved-thawed testicular spermatozoa.</p><p><b>RESULTS</b>A total of 35 couples underwent 35 ICSI cycles using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration. The clinical pregnancy rate was 37.14% (13/35).</p><p><b>CONCLUSION</b>ICSI using cryopreserved-thawed testicular spermatozoa with testicular fine needle aspiration is a main and effective method in the treatment of non-obstructive azoospermia, which can avoid further testicular fine needle aspiration.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Pregnancy , Biopsy, Fine-Needle , Cryopreservation , Oligospermia , Pathology , Therapeutics , Semen Preservation , Sperm Injections, Intracytoplasmic , Methods , Testis , Pathology , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To study the polymorphisms of DYS287 and DYS440 in Zhejiang She population.</p><p><b>METHODS</b>Detection of DYS287 and DYS440 polymorphisms was performed in 100 She individuals by using polymerase chain reaction amplification and 2% agarose gel electrophoresis.</p><p><b>RESULTS</b>With 150 bp product in all She individuals, YAP(+) was absent. DYS440*3 was present in 10 She individuals (10%); DYS440*4 was present in the other 90 She individuals(90%).</p><p><b>CONCLUSION</b>The polymorphisms of DYS287 and DYS440 in Zhejiang She population were different from those in the other populations that belong to Sino-Tibetan Language Family. So both DYS440 and DYS287 are important and stable genetic markers, they can provide reliable evidence in human evolution study.</p>