ABSTRACT
OBJECTIVE:To establish a method for simultaneous determination of ethinylestradiol and norelgestromin in hor-monal patches. METHODS:RP-HPLC method was performed on column of WondaCract ODS-2 with mobile phase of metha-nol-0.5% phosphoric acid(68∶32,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 220 nm,the detection tempera-ture was 25 ℃,and sample volume was 20 μl. RESULTS:The linear relationship was 0.872-130.8 μg/ml for ethinylestradiol(r=0.999 6) and 0.880-132.0 μg/ml for norelgestromin (r=0.999 7);RSDs of precision,reproducibility and stability tests were no more than 1.03%,average recoveries were resectively 101.50%(RSD=0.99%,n=9) and 101.68%(RSD=0.95%,n=9). CON-CLUSIONS:The established method is simple and accurate,and can be used for the content determination of ethinylestradiol and norelgestromin in hormonal patches.
ABSTRACT
Objective: To study the protective effects of polypeptide-Fe on acute hepatic injury induced by CCl4 in mice. Method:Mice model of acute hepatic injury was set up by CCl4 ig (50mg/kg?d ). and fed with polypeptide-Fe 67.5 mg/kg?d, 675 mg/kg?d and 2 025 mg/kg?d for 30 d respectively. The effects on serum ALT, AST, and ALP and liver TG, TC, GSH, MDA, and SOD avtivities were observed. Results: Polypeptide-Fe decreased the activities of ALT, AST, and ALP in serum and the levels of TG, TC, and MDA in liver, and increased the level of GSH in liver. Middle dosage group of polypeptide-Fe was the most effective one. Conclusion:Polypeptide-Fe showed significant antioxidant ability in protecting acute hepatic injury induced by CCl4 in mice.