ABSTRACT
High mobility group protein B1 (HMGB1) is encoded by HMGB1 gene and is a highly conserved non-histone chromosomal binding protein widely present in the nucleus. It plays an important role in DNA replication and repair of eukaryotic cells. As a delayed inflammatory mediator, HMGB1 secreted or released extracellularly mediates inflammatory response in sepsis, autoimmune diseases, malignant tumors, liver-related diseases, and so on. In recent years, the studies of HMGB1 gene polymorphism have provided a theoretical basis for further clarifying the pathogenesis of diseases. These studies provided early predictions of disease susceptibility for early intervention and provided the basis for precise prevention and treatment of diseases. In this paper, the correlation between HMGB1 and clinical disease susceptibility and prognosis was reviewed.
ABSTRACT
[Objective] To establish a method for determination of Ephedrine Hydrochloride in KMBYL capsules by HPLC.[Methods] The DiamonsilTM C18(250mm?4.6mm,5 ?m)column was used.The mobile phase was mobile phase: acetonitrine-0.02 mol?L-1potassium dihydrogen phosphate(0.2% triethylamine,adjusteding pH to 2.7 with phosphoric acid)(5∶95) with a flow rate of 1.0ml?min-1.The detection wavelength was 210 nm.The column temperature was 30℃.[Results] The linear responses range was 0.2148-2.148 ?g?mL-1 of Ephedrine Hydrochloride(r=0?9999).The average recovery was 98.85% and the RSD was 2.95%.[Conclusion] The method is simple accurate reproducible.It can be used for the quality control of KMBYL Capsules.