ABSTRACT
Objective To understand prevalence and transmission of transmitted drug resistance (TDR) among HIV infected men who have sex with men (MSM) in Tianjin from 2014 to 2017.Methods A total of 225 blood samples were collected from HIV infected MSM in Tianjin from 2014 to 2017.Pol gene fragments were obtained by viral RNA extraction and nested PCR amplification.Phylogenetic and drug resistance analyses were conducted.Results A total of 205 samples were successfully sequenced and analyzed.Based on pol sequences,53.2％ (109/205),28.8％ (59/205),10.2％ (21/205),4.9％ (10/205) and 2.9％ (6/205) of the samples were positive for HIV subtypes CRF0 1_AE,CRF07_BC,B,CRF55_01B and unique recombinant forms (URFs).Twenty transmission clusters,including 75 sequences,were identified and 62.5％ (10/16) of sequences with TDR were in 5 clusters.The prevalence of TDR was 7.8％ between 2014 and 2017.The annual prevalence rate increased from 3.9％ (2/51) in 2014,5.7％ (3/53) in 2015,9.6％ (5/52) in 2016 to 12.2％(6/49) in 2017,the difference was not significant (x2=2.504,P=0.127).CRF01_AE and B strains had high TDR prevalence (3.4％,7/205) and (2.9％,6/205),respectively.The TDR mutation was mainly NNRTIs,the TDR prevalence was 6.3％ (13/205).In contract,the TDR prevalence of NRTIs and PIs were 1.5％ (3/205) and 1.0％ (2/205) respectively.Conclusion Results from this study suggested that the prevalence of HIV-1 TDR strains in MSM was serious in Tianjin.It is necessary to take effective prevention and control measures.
ABSTRACT
Objective To understand prevalence and transmission of transmitted drug resistance (TDR) among HIV infected men who have sex with men (MSM) in Tianjin from 2014 to 2017.Methods A total of 225 blood samples were collected from HIV infected MSM in Tianjin from 2014 to 2017.Pol gene fragments were obtained by viral RNA extraction and nested PCR amplification.Phylogenetic and drug resistance analyses were conducted.Results A total of 205 samples were successfully sequenced and analyzed.Based on pol sequences,53.2％ (109/205),28.8％ (59/205),10.2％ (21/205),4.9％ (10/205) and 2.9％ (6/205) of the samples were positive for HIV subtypes CRF0 1_AE,CRF07_BC,B,CRF55_01B and unique recombinant forms (URFs).Twenty transmission clusters,including 75 sequences,were identified and 62.5％ (10/16) of sequences with TDR were in 5 clusters.The prevalence of TDR was 7.8％ between 2014 and 2017.The annual prevalence rate increased from 3.9％ (2/51) in 2014,5.7％ (3/53) in 2015,9.6％ (5/52) in 2016 to 12.2％(6/49) in 2017,the difference was not significant (x2=2.504,P=0.127).CRF01_AE and B strains had high TDR prevalence (3.4％,7/205) and (2.9％,6/205),respectively.The TDR mutation was mainly NNRTIs,the TDR prevalence was 6.3％ (13/205).In contract,the TDR prevalence of NRTIs and PIs were 1.5％ (3/205) and 1.0％ (2/205) respectively.Conclusion Results from this study suggested that the prevalence of HIV-1 TDR strains in MSM was serious in Tianjin.It is necessary to take effective prevention and control measures.
ABSTRACT
Objective To evaluate the auxiliary diagnostic value of viral load test for samples with indeterminate HIV antibody detection results.Methods Thirty-two samples with indeterminate HIV antibody detection results collected from MSM were used for two viral load tests in Tianjin in 2015.Follow up was conducted for this population to confirm their HIV infection status.Results Virus loads were detected in the 2 viral load tests.In the follow-up survey,the results of HIV antibody test of all the samples were positive.The results of viral load test were completely consistent with those of HIIV antibody confirmation test.Conclusion The viral load test was effective in the auxiliary diagnosis of HIV infection in window phase,which can be used for the samples with indeterminate HIV antibody detection results.
ABSTRACT
Objective To evaluate the auxiliary diagnostic value of viral load test for samples with indeterminate HIV antibody detection results.Methods Thirty-two samples with indeterminate HIV antibody detection results collected from MSM were used for two viral load tests in Tianjin in 2015.Follow up was conducted for this population to confirm their HIV infection status.Results Virus loads were detected in the 2 viral load tests.In the follow-up survey,the results of HIV antibody test of all the samples were positive.The results of viral load test were completely consistent with those of HIIV antibody confirmation test.Conclusion The viral load test was effective in the auxiliary diagnosis of HIV infection in window phase,which can be used for the samples with indeterminate HIV antibody detection results.