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Objective To investigate the performance of MicroScan WalkAway 96 Plus (MSW) system in detection of carbapenem-resistant Enterobacteriaceae (CRE).Methods A total of 81 stock CRE strains were used in this study. Bacterial identification and antimicrobial susceptibility test were performed by MSW system. Beta-lactamases genes blaKPC,blaIMP,blaVIM, blaOXA-48 and blaNDM were amplified by PCR and subjected to sequencing analysis. Disk diffusion method and PCR were used as gold standard to evaluate the performance and reliability of MSW system in identifying carbapenem-resistant and carbapenemase-producing Enterobacteriaceae.Results Overall, 69.1 % (56/81) of the Enterobacteriaceae strains were identified as CRE by the MSW system. The results of PCR showed that 48 strains were carbapenemase-producing Enterobacteriaceae. When carbapenemase-producing Enterobacteriaceae strains were identified by the instrument using an advanced expert system, the sensitivity was 93.8 % and specificity was 42.4 %. The positive predictive value was 70.3 %, the negative predictive value was 82.4 % and the predictive accuracy value was 72.8 %.Conclusions The MicroScan WalkAway 96 Plus system has shown good performance in detection of CRE.
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Objective To observe the analgesic effects of tramadol combined with atropine during the oocyte retrieval operation in assisted reproduction treatment(IVF/ICSI-ET)? Methods Three hundred patients(four hundred cycles of infertility totally)in the Center for Reproductive Medicine were equally assigned into treatment group and control group according to the different analgesia: the former were treated with intramuscular injection of 100 mg tramadol combined with 0?5 mg atropine for analgesia before the operation and the latter with 50 mg pethidine hydrochloride? The two groups were compared in terms of blood pressure,pulse,degree of pains, rate of fertility,rate of cleavage and rate of transplantable embryo? Result There were no differences between them in terms of blood pressure,pulse,pain degree,rate of fertility and rate of cleavage and rate of transplantable embryo(all P > 0?05),but the incidence of adverse effect in the treatment group was significantly lower than that in the control group(P < 0?05)? Conclusion The application of tramadol hydrochloride combined with atropine sulphatev for analgesia during oocyte retrieval operation of IVF-ET is advantageous for its safety,lower incidence of advers effect and stable vital signs?
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10.3969/j.issn.2095-4344.2013.25.013
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Foreign bodies in the paranasal sinuses are rare. Foreign bodies in the ethmoid sinus are easily misdiagnosed as tumor. There is no typical display in CT and MRI tests. The described case showed the misdiagnosis of an ethmoid foreign body as tumour on the basis of CT and MRI results. It should be emphasised that the tumour diagnosis should be based on further physical and histopathological examinations and not only on the results of CT and MRI tests.
Subject(s)
Adult , Humans , Male , Diagnostic Errors , Ethmoid Sinus , Foreign Bodies , Diagnosis , Paranasal Sinus Neoplasms , DiagnosisABSTRACT
The A/G polymorphism of cytotoxic T lymphocyte associated antiger 4(CTLA-4) was determined by PCR-RFLP in 120 patients with recurrent Graves′ disease (GD), including 85 cases with early relapses within 3 years and 35 cases with relapses more than 3 years after withdrawal of antithyroid medication.66 initial GD patients and 100 healthy controls were included in the present study.The results suggest that A/G polymorphism of CTLA-4 was associated not only with GD, but also with early relapes after withdrawal of antithyroid medication.
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AIM: To observe the efficacy and side effects of interferon ? 1b (interferon) combined with oxymatrine in the treatment of chronic hepatitis B. METHODS: 87 patients were randomly divided into two groups: 45 patients in treatment group were given interferon 30 ?g?d -1 (im) for 4 wk, then 30 ?g?d -1 (im, qod) combined with oxymatrine 600 mg?d -1 (im) for 3 mon; 42 patients in control group were given the same dose of interferon as above only. After completion of therapy, liver function and markers of HBVM were compared between the two groups. RESULTS: The rates of ALT decreased to normal range in treatment group and control group were 88.9 % and 60%, respectively (P