ABSTRACT
Virus inactivation with photochemistry is being suitable for blood or blood products, methylene blue (MB)/light treatment has been used for viral inactivation of cellular blood components. Twelve new phenothiazines derivatives were designed and synthesized, and were used to test viral inactivation and red cell damage preliminary. Results showed that compound YWW-7 has a satisfactory activity, it could be developed as a new viral inactivation agent for blood products.
ABSTRACT
Objective To research the effect of Hydroxyl radical(type Ⅰ mechanism)and singlet oxygen(type Ⅱ mechanism)on virus inactivation and red cell cytotoxicity induced by DMMB and M007 phototreatment.Methods Hydroxyl radical quenchers mannitol and glycerol,singlet oxygen quenchers sodium azide and tryptophan were employed to investigated their ability to inhibit the inactivation of bacteriophage Phi6 and red cell cytotoxicity by induced DMMB and M007 phototreatment.Results Singlet oxygen quenchers could inhibit the inactivation of bacteriophage Phi6 obviously, whereas hydroxyl quenchers hardly affect the inactivation of bacteriophage Phi6.Both type of quenchers could reduce the hemolysis caused by CMMB or M007 phototreatment.Conclusion Viral inactivation of DMMB and M007 phototreatment is mainly through type Ⅱ mechanism, both type I and type Ⅱ mechanisms contribute to red cell cytotoxicity.
ABSTRACT
The increase in the maximum time of collected whole blood storage at room temperature from 8 to 24 hours should bring about the versatile benefits. For making the mentioned above change it was necessary not only to demonstrate the blood components prepared from the whole blood stored at room temperature for 24 hours should still keep their functions, but also to indicate that the increase in the time of whole blood storage at room temperature couldn't accelerate the bacterial proliferation. Therefore, the two units of whole blood were pooled,inoculated with bacteria, split again into two units,stored at room temperature for 8 and 24 hours, respectively,and then the blood components were separated. The levels of the bacteria found in the blood components were measured in the process of their storage, indicating that the whole blood stored at room temperature for 24 hours couldn't accelerate the proliferation rate of 5 common contaminating bacteria existed in the blood components in their storage process.
ABSTRACT
The ?_2-macroglobulin(?_2M)pre- paration was pasteurized,using 40g/dl of xylitol as a stabilizer,to reduce the risk of transmission of the viral diseases such as AIDS and hepatitis during the clinical trans- fusion.The ?_2-M preparation,treated by heating at 60℃ for 10 hours, can still preserve fully its trypsin- binding biological activity and im- munological reactivity,and no new antigens were detected,which had the behavior in eleetrophoresis and the catabolic rate in rabbit that were not different from those of the un- heated samples,indicating that the pasteurized ?_2-M preparation still keeps its good native properties.
ABSTRACT
An intravenous immunoglobulin preparation(IVIG)in solution was pasteurized,or heated at 60℃ for 10 hours in the presence of 50g/dl xylitol as stabilizer.The results show- ed that the antibody levels against diphtheria,measles viruses and HBs Ag,of the pasteurized IVIG,were not changed,the Fc function of IgG molecule was not damaged,the anti- complement activity was reduced significantly,and its stability was improved.Both model viruses,the cytomegalovirus and the simian retrovirus D1,added in the prepara- tion,were inactivated rapidly under the above-mentioned conditions as those of IVIG pasteurization.It was suggested that the pasteurized IVIG preparation not only fully saved its biological activity,but also would be better tolerance for intravenous injection and the safety in preven- tion of viral disease transmission.
ABSTRACT
9.9Log10 bacteriophage R 17 Conclusion Alkylation of the phenothiazine ring increased the potential of inactivating virus and decreased the damage to red blood cells? M007 might be an alternative phenothiazine dye that can be used to inactivate viruses in red blood cells?