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BACKGROUND: There is no systematical report on the conserved structure, protein characteristics and phylogenetic relationship of the Fasciclin gene family. OBJECTIVE: To investigate the evolutionary history of the Fasciclin gene family, and to systematically analyze the conservative structure, protein characteristics, and phylogenetic relationships of this gene family METHODS: Based on the published genome data of eight species such as people, chimpanzee and zebrafish, HMM models were established using the HMMER software to analyze the characteristics and genetic structure, structure domain and biochemical characteristics, evolutionary relationships and expression characteristics of the Fasciclin gene family. RESULTS AND CONCLUSION: In the eight species of fish (zebrafish), amphibian (xenopus), bird (chicken), reptile (Trionyx sinensis), mammal (rat, mouse) and primate (chimpanzee, human), 5, 5, 4, 5, 5, 4, 4, and 4 fasciclin candidate genes were retrieved respectively. Through the phylogenetic analysis of the fasciclin gene in these eight species, the evolutionary tree of fasciclin gene families can be divided into four evolution branch. The two branches of all are more conservative, likely to be the main osteoblast specific factor; the third branch may be osteoblast specific factor of amphibians, mammals and primate; and the final branch also has a good conservative, but the Xenopus and mouse in the branch has two copies of the genes, and this branch may play an important role in the evolution of these two species. Overall, in the evolution of species, the gene family has no larger changes, and such genes may be important for the growth and development. The lack of such genes may lead to the death of the individual or the extinction of species.
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Objective To investigate the potential risk factors for mild cognitive impairment (MCI) in the elderly population in the community, and to provide a basis for the primary prevention of MCI. Methods A cross-sectional study of elderly population in communities of Shanghai, China was conducted. A total of 368 subjects including both males and females, aged 65-80 years old, were selected to complete the mini-mental state examination (MMSE), basic information questionnaires, and physical examinations. Logistic regression analysis was used to analyze the potential risk factors of MCI. Results Of the 368 subjects participating in the study, 53 were found to have MCI and the prevalence rate was 14.4%. Univariate analysis found that older age, low education, no folic acid supplementation, stroke, osteoporosis and hyperlipidemia were risk factors of MCI. Multiple logistic regression analysis showed that advanced age [OR=1.146 (95%CI: 1.052-1.249)] and osteoporosis [OR=2.371 (95%CI: 1.042-5.396)] were the independent risk factors for MCI, while higher education [OR=0.073 (95%CI: 0.011-0.478)] was a protective factor. Age influenced all the aspects of MMSE scores (all P values <0.05). In addition, the analysis of the results suggested that subjects with regular folic acid supplementation got higher MMSE scores, especially in the aspect of language and praxis (P=0.002). On the contrary, patients with osteoporosis had lower attention and computing power scores (P=0.022). Conclusion The prevalence of MCI increased with age. Low education and osteoporosis may be the independent risk factors for MCI in the elderly population. Although no association was observed between folic acid supplementation and MCI, folic acid supplementation could improve the performance of language and praxis.
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OBJECTIVE： To provide reference for clinical treatment of precancerous lesions of gastric cancer. METHODS： A total of 685 patients with precancerous lesions of gastric cancer were selected from our hospital during Jan.-Dec. 2018. Totally 455 patients in treatment group received individualized TCM therapy according to syndrome differentiation， 7 days as a course， 4 courses in total； another 230 patients in control group received Folic acid tablets， 5 mg， tid， for 2 months. The changes of pepsinogen ratio （PGⅠ/PGⅡ）， gastrin-17 （G-17） and Helicobacter pylori （Hp）before and after treatment， and the occurrence of ADR were compared between 2 groups. RESULTS： After treatment， total response rate of treatment group was 90.3％， which was significantly higher than 68.6％ of control group. PGⅠ/PGⅡ of treatment group were increased significantly， which was significantly higher than control group； G-17 levels of 2 groups were decreased significantly （P＜0.05）； there was no statistical significance in Hp level between 2 groups before and after treatment （P＞0.05）. There was no statistical significance in the incidence of ADR between 2 groups（P＞0.05）. CONCLUSIONS： Chinese medicine syndrome differentiation individual therapy can significantly delay the development of precancerous lesions of gastric cancer， and has good safety.
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Objective To observe the effect of curcumin combined with cisplatin on the proliferation of human tongue squamous cell carcinoma Tca?8113 cells,and explore the possible mechanism of their anti?tumor effect in vitro. Methods In this study,the samples were divided into four groups:curcumin,cisplatin,curcumin combined with cisplatin,and control. Detection of rate of inhibition of Tca?8113 cell proliferation was carried out by MTT assay. This was followed by observation of the morphological changes of the nuclei by Hoechst 33258 fluorescence staining. Subse?quently,cellular apoptosis was assayed by flow cytometry,and expression of Notch1 and epidermal growth factor receptor(EGFR)was examined by Western blotting. Results Results of MTT assay showed that curcumin combined with cisplatin inhibited the proliferation of tongue squamous cell carcinoma to a greater extent than either curcumin or cisplatin alone(P<0.05). Flow cytometry analysis indicated that,unlike curcumin or cisplatin alone,curcumin combined with cisplatin noticeably induced apoptosis(P<0.05). Results of Western blotting revealed that the expres?sion of Notch1 was upregulated,whereas the expression of EGFR was downregulated to a greater extent in the control group than in cells treated with curcumin or cisplatin alone. Unlike curcumin and cisplatin groups,the combined group revealed statistically significant expressions of Notch1 and EGFR(P<0.05). Conclusion Curcumin and cisplatin have a combined effect on inhibition of proliferation of human tongue squamous cell carcinoma Tca?8113 cells. The mechanism may be related to upregulation of the Notch1 signaling pathway and downregulation of the EGFR signal?ing pathway.
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Objective:To study the exogenous oxidative stimuli on the mtDNA damage and proliferation of oral cancer SCC-25 cells.Methods:SCC-25 cells and fibroblast cell line BJ cells were treated by H2O2 at 0 (the control),120,240,480 and 960 μmol/L respectively for 15 m-24 h respectively,mtDNA damage was measured by a supercoiling-sensitive qPCR method(ss-qPCR),cell proliferation was tested by MTT assay.Results:Dose-dependent mtDNA damage was observed in SCC-25 cells treated by H2O2 at 120 μmol/L or above,and in BJ cells by H2O2 at 240 μmol/L or above,the mtDNA damage rate of both cells decreased 2-24 h after H2O2 treatment,in the 120 μmol/L group the damage rate reached to the control group.BJ cells showed less reaction to H2O2 stimulation than SCC-25 cells.Conclusion:Oxidative stress may be related to the development of oral cancer.
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Objective To explore the effects of group sand play on the behavior of cerebral palsy (CP)children with behavioral problems.Methods Ninety children with CP aged 3-5 with behavioral problems were selected,after those with mental retardation had been excluded through the Peabody picture vocabulary test (PPVT).Their behavioral problems were diagnosed using Achenhach's child behavior check list (CRCL).The children were randomly divided into an experimental group and a control group with 45 cases in each.The experimental group received 10 weeks of sand play treatment in addition to routine rehabilitation.The controls received only routine rehabilitation.Results After treatment,the social withdrawal,depression,aggression and disruptive behavior scores of the 3-year olds in the experimental group were significantly lower than before treatment and significantly lower than those of the controls.For the 4 and 5 year-old boys the results were similar in terms of soclal withdrawal,depression,immaturity,sexual behavior,aggression and delinquent behavior,For the 4 and 5 year-old girls the scores for depression,somatic complaints,social withdrawal,compulsion,aggression,and hyperauctivity showed similar significant improvements.Conclusion Group sand play can improve the behavior and promote the mental health of CP children.