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@#The PI3K signaling pathway is frequently over-expressed in a variety of hematologic malignancies, so the development of PI3K inhibitors for the treatment of hematologic malignancies has broad application prospects. In this study, a novel PI3K inhibitor, JN-65, was identified through the investigation effects in the inhibition to hematologic malignancies. By MTT assays, JN-65 was found to effectively suppress the proliferation of hematologic malignancies, especially leukemia cell lines. The cell-free enzymatic studies demonstrated that JN-65 cloud inhibit PI3K and specifically inhibited PI3Kγ at low micromolar concentrations. Western blot confirmed that JN-65 could effectively inhibit the PI3K/Akt signaling pathway and the flow cytometry assays verified that JN-65 could induce the apoptosis of tumor cells through the suppression of PI3K signaling pathway. Finally, the molecular docking simulation method was used to explore the interaction between JN-65 and PI3K, and the inhibition mechanism of PI3K was revealed at the molecular level. In general, JN-65 would be a potential PI3K inhibitor for the treatment of hematologic malignancies.
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Objective:To investigate the factors and mechanisms in forming uric acid stones in patients with type 2 diabetes (T2DM).Methods:106 patients with diabetes were divided into observation group and control group according to the combination of urinary calculi,53 cases in each group,The differences of clinical data and biochemical indexes between the two groups were compared,The relationship between type 2 diabetes mellitus and urinary stones was analyzed by multi factor regression analysis.Results:There were no significant difference in observation group and control group in age,sex,SBP,DBP,TC,FBG,2hPBG and HbA1C (P＞0.05),and there were of statistical difference significance in BMI,urinary pH,HOMA-IR,SUA,TUA in the two gruops (P ＜0.05) and the Logistic regression analysis showed blood uric acid,the urinary pH,HOMA-IR,SUA were independent risk factors in urolithiasis in T2DM (P ＜ 0.05).Conclusion:High uric acid hematic disease,high uric acid excretion,insulin resistance,overweight or obesity,high blood triglycerides in patients with type 2 diabetes is risk factors for urinary stone formation,in which blood uric acid,urinary pH,HOMA-IR is the independent risk factor for type 2 diabetic patients with urinary calculi.
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@#Objective To observe the effect of electroacupuncture on nerve root of sacral spinal nerve 3 for detrusor hyperreflexia after spinal cord injury. Methods 32 spinal cord injury patients with detrusor hyperreflexia accepted electroacupuncture at sacral 3 nerve root.Their voiding diary, urodynamics and quality of life were observed before and 8 weeks after treatment. Results The frequency of urination,urine leakage, and filling the final detrusor pressure decreased (P<0.05), and the urine output, maximum bladder capacity, bladder compliance,score of quality of life increased (P<0.05) after treatment. There was no significant change in residual urine volume after treatment (P>0.05). Conclusion Electroacupuncture on nerve root of sacral spinal nerve 3 is effective on detrusor hyperreflexia after spinal cord injury.
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@#Objective To observe the effect of sigmoid colon enterocystoplasty on urge incontinence of neurogenic bladder. Methods 16 patients with urge incontinence without bladder-ureter reflux or hydronephrosis accepted the sigmoid colon enterocystoplasty. They were followed up for 3~24 months. Results No bladder-ureter reflux and hydronephrosis was found. The biochemical parameters were stable. Constipation relieved. All the patients could urinate with abdominal pressure and no urge incontinence happened. The residual urine volume was 10~40 ml. Conclusion Sigmoid colon enterocystoplasty is effective on urge incontinence of neurogenic bladder, as well as on constipation.
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@#ObjectiveTo observe the effect of third sacral nerve root electro-acupuncture on acontractile detrusor. Methods84 patients with acontractile detrusor were divided into 2 groups received urethral catheterization alone (control) or electro-acupuncture on third sacral nerve (treatment) respectively. The voiding diary (including urination times, nocturia times and urinary volume) and urodynamics test (including maximum bladder volume, maximum systolic pressure detrusor, the effective time of detrusor contraction, maximum urinary flow rate, average urinary flow rate and residual urine volume) were observed before and 8 week after treatment. ResultsCompared with those before treatment, the urination times, nocturia times and residual urine volume decreased (P<0.05) in treatment group, while maximum systolic pressure detrusor, the effective time of detrusor contraction, maximum urinary flow rate, average urinary flow rate and residual urine volume improved (P<0.05), but the maximum bladder capacity showed no significant difference (P>0.05). ConclusionElectro-acupuncture on third sacral nerve is an effective intervention for acontractile detrusor.
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Objective To investigate effectiveness of treatment of Upper urinary tract calculus by MPCNL application.Methods From May 2000 to May 2007,causing artificial kidney hydronephrosis for the affected kidney,established renal percutaneous channels(F16～18),which established the single channels of 202 cases,dual-channel of 14 cases,by Ureteroscopic Lithotripsy and holmium laser lithotripsy.Results 183 cases of 216 patients were One-time stone;33 cases were second stone.The average clearance rate is 90.32%,the average operation time is 79min,average valume of about bleeding is 80ml,the average hospitalization days are 8 days,kidney ostomy average stay 7.5 days.Of the operation,9 cases of obviously bleeding(4%)before and after operation,1 case uncontrollable bleeding after interventional treatment failure with Nephrectomy,others to stop beeding by symptomatic treatment,2 cases find urinary extravasation postoperative and cured by adjusting location of renal ostomy.Conclusion Minimally invasive percutaneous nephrolithotomy for the treatment of Upper urinary tract calculus is safe and effective for patients with less trauma,quicker recovery and high learance rate,the efficacy is satifactory.