ABSTRACT
Objective To investigate the effect mechanism of sanguinarine on the proliferation,apoptosis,invasion and migration abilities of cervical cancer cells.Methods MTT,flow cytometer,cell scratch test and Transwell chamber assay were respectively used to detect the cellular proliferation,apoptosis,migration and invasion abilities after sanguinarine action.The expression levels of E-Cadherin,PTEN,β-catenin and MMP2 protein of cervical cancer cells after sanguinarine action were detected by Western blot.Results 0.6,0.8 μmol/L sanguinarine had the inhibitory effect on the proliferation of cervical cancer cells.After 0.8μmol/L sanguinarine action for 48 h,cervical cancer HeLa and Siha cells apoptosis rate were up to (45.68± 2.26)％ and(31.89 ± 3.80)％ respectively.0.8 μmol/L sanguinarine action for 3 h,cervical cancer cells HeLa and Siha adhesion rates were only (67.45 ± 2.13)％and(73.59± 2.61)％.0.8 mol/L sanguinarine action for 16 h,the invasion numbers of cervical cancer Hela and Siha cell were (39.64 ±1.98) and (43.87 ± 2.83) respectively.The expression amount of E-Cadherin and PTEN in cervical cancer cells after sanguinarine action was increased,while the expression amount of E-Cadherin and PTEN was weakened.Conclusion Sanguinarine has the proliferation inhibiting and apoptosis promoting effect on cervical cancer cells,its mechanism may be related to adhesion protein E-Cadherin,β-catenin and PTEN,MMP2.
ABSTRACT
BACKGROUND:Bare-metal stent for treatment of myocardial infarction may improve cardiac function to some extent, but it is prone to induce thrombus. OBJECTIVE:To explore the effect of heparin slow-release stent on angiogenesis and cardiac function of patients with acute myocardial infarction. METHODS:A total of 87 patients with acute myocardial infarction were enroled, including 47 males and 40 females, aged 55-81 years. These patients were divided into observation group (n=45) with percutaneous coronary intervention with heparin slow-release stent and control group (n=42) with percutaneous coronary intervention with bare metal stent. The folow-up period was 12 months, and angiogenesis, cardiac function improvement and adverse cardiac events in the two groups were observed and compared. RESULTS AND CONCLUSION:The new blood vessel density, myocardial survival area and left ventricular ejection fraction were al higher in the observation group than the control group (P < 0.05). At 3 months after intervention, there was one case of revascularization and one case of recurrent acute myocardial infarction in the observation group; in the control group, there was case of revascularization, one case of recurrent acute myocardial infarction and one case of sudden cardiac death, and there was no difference between the two groups. These findings indicate that that percutaneous coronary intervention with heparin slow-release stent has good biocompatibility and can effectively promote the regeneration of blood vessels and improve cardiac function of patients with acute myocardial infarction.