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OBJECTIVE:To establish a method for content determination of cyproheptadine hydrochloride in fuyan cream.METHODS:Ultraviolet spectophotometry was applied to determine the contents of cyprohepatadine hydrochloride as well as its adjuvant as two coexisting components without isolation and extraction,the wavelengths were286nm and258nm res_ pectively,linear regression and simultaneous equations were used to analyze the results.RESULTS:Cyproheptadine hydrochlor_ ide and its adjuvant showed good linear relationship in the range of2.52～25.2and2.5022～25.022?g/ml respectively,the average recovery of cyproheptadine hydrochloride was98.61%(RSD=1.08%).CONCLUSION:The present method is simple,convenient,reproducible and reliable,which is suitable for rapid determination for fuyan cream.
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<p><b>BACKGROUND</b>To investigate the clinical results of radio-frequency ablation in the treatment of locally advanced lung cancer.</p><p><b>METHODS</b>Twenty-two patients with locally advanced lung cancer were treated with radio-frequency ablation guided by CT.</p><p><b>RESULTS</b>CT scan after radio-frequency ablation showed that there was cavity in the parenchyma of cancer. Most lung cancer patients felt remittent in chest pain in some extend. Follow-up CT scan in 20 patients revealed retraction of the treated region 3 months later after radio-frequency ablation. Out of the 20 patients, 1 had complete response, 12 had partial response, 4 had minor response, and 3 had stable diseases.</p><p><b>CONCLUSIONS</b>Radio-frequency ablation guided by CT is a promising minimally invasive technique in the treatment of locally advanced lung cancer.</p>
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Objective To observe the characteristic, mechanisms and the change of pathophysiology about thoracic explosive injury from an explosive device. Methods Seventy-three millimetres prefabricated steel-ball bomb was used to be the center of explosion. According to the distance from each group to the center of explosion, 30 sheep were randomly divided into 5 groups. The injuries of thoracic wall, lung and surrounding organs were observed from fragments and blast wave. Results The mortality of thoracic explosive injury on the ground testing was 56.7%.Most of fragment wounds were blind wound. The track of wounds was often complicated and curved. The major associated injuries were myocardial contusion, splenic-hepatic rupture and gastric-enteric rupture. There were much severe pulmonary hemorrhage and lung edema. The parameters of trauma were well correlated with the severity of thoracic injury. Conclusion The parameters of trauma were well correlated with the severity of thoracic injury. The characteristic of thoracic injury from explosive device was more complicated and severe.
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Objective To establish a novel liver and lung metastatic tumor model in nude mice by splenic inoculation of hilar cholangiocarcinoma cell line.Methods Human hilar cholangiocarcinoma cell line(FPH-0201) cells was established and cultured with a fresh human hilar cholangiocarcinoma specimen.The spleen of 7 nude mice was injected with 0.1ml of hilar cholangiocarcinoma cell suspension(1.5?10~7 cell).The tumor appearing time,tumor local and metastatic formation rate,histological,morphological and ultrastructure of metastatic tumor cell were observed.The sub-cell line was obtained by cutture of metastatic specimen,and sub-cell line cells were again injected into the lower pole of the spleen and metastatic tumor formation rate was observed again.Results The overall local tumor formation rate in spleen was 100%,and metastatic tumor formation rate was 14.3%.The sub-cell line cells that were re-inoculated in mice spleen,had a metastatic tumor formation rate of 100%.Morphological observation of metastatic tumor showed the common features of cholangiocarcinoma.Chromosome number showed a variation of 18~44.Conclusions The hilar cholagiocarcinoma metastatic tumor model that was established in this laboratomy had characteristics compatible with malignant tumor and had all the biological characteristics of human hilar cholagiocarcinoma.