ABSTRACT
Objective To investigate the injury death and its potential years of life lost among residents in Changsha from 2014 to 2021. Methods Data was obtained from the routine death cause monitoring records, and descriptive analysis was conducted using SPSS 21.0 to analyze the characteristics of injury death and life loss. Joinpoint software was applied to analyze and compare the mortality rates of injury death causes in different years. Results From 2014 to 2021, a total of 23862 deaths from injuries were reported in residents in Changsha, with an average annual crude mortality rate of 37.23/105 and an average standardized mortality rate of 33.82/105. The age standardized rate in the male was higher than that in the female (χ2＝2496.29，P=0.000). The standardized mortality rate of injury decreased year by year (APC=-4.013%，t=-3.052，P=0.022). The top five causes of death from injurie were traffic accident, accidental fall, drowning, suicide, and accidental poisoning. The leading causes of injury death were drowning in the age group of 0 to 14 years old, traffic accident in the group of 15 to 64 years old, and accidental fall in the group of 65 years old and above. From 2014 to 2021, the cumulative potential years of life lost (PYLL) of residents in Changsha was 398280.00 person years, the average years of life lost (AYLL) was 23.82 years per person, and the potential years of life lost rate (PYLLR) was 6.68‰. The top 5 types of injuries for PYLL were traffic accident, drowning, accidental fall, suicide, and accidental poisoning. Conclusion Injury is one of the important public health problems affecting the health of the residents in Changsha, and corresponding intervention measures should be taken according to the injury types of different populations.
ABSTRACT
Background and purpose: Colorectal cancer (CRC) is the most frequently occurring primary malignant tumor. Chemotherapy can reduce the risk of local and distant relapse. Therefore, it is very important to ifnd new biomarkers that can predict chemoresistant and help in treatment decisions. Methods:In this study, we examined the expression levels of 1 200 human miRNAs in 6 CRC tissues, using miRNA proifling assay arrays. A validation study was done to corroborate a subset of the results, including expression levels of miR-4299, miR-196b, miR-324-5p, miR-455-3p and miR-939, by analyzing 100 specimens of stageⅣcolorectal adenocarcinoma (not respond and respond to the chemotherapy) to quantitative real-time PCR. We modeled the relationship between the expression levels of these miRNAs and the survival rate of 100 CRC patients by Kaplan-Meier method. Results:Expression proifles in CRCs suggested that 5 miRNAs were candidate markers associated with the chemoresistance of colorectal cancer. We found that miR-4299 and-196b had signiifcant diagnostic value for chemoresistance CRC. miR-4299 yielded an AUC (the areas under the ROC curve) of 0.784 and miR-196b yielded an AUC of 0.647 in discriminating CRC from controls. Combined ROC analysis using these 2 miRNAs revealed an elevated AUC of 0.848 with 67.9%sensitivity and 90.9%speciifcity in discriminating chemoresistance CRC. The low level of miR-4299 expression and the high level of-196b expression are signiifcantly correlated with the good survival of CRC patients. Conclusion:These data suggest that miR-4299 and-196b have strong potential as novel biomarkers for chemoresistant detection of colorectal cancer.
ABSTRACT
Objective To detect the inhibitory effect of HSP27-siRNA on hepatocellular carcinoma(HCC) cells.Methods HCC cell QGY lines were cultured with HSP27-siRNA in a differtent range of concentration for various time periods.Cell activity was studied by MTT.The changes of cell cycle and apoptosis were analyzed by FCM.RT-PCR was used to detect the effect of HSP27-siRNA on QGY cell expression of HSP27 mRNA.Western blot was used to detect the inhibition efficiency of HSP27-siRNA on HSP27protein.Results The proliferation of QGY cell was inhibited by HSP27-siRNA,and HSP27-siRNA decreased the expression of HSP27 protein.HSP27-siRNA inhibited the proliferation of QGY cell line and induced apoptosis in vitro,and its effect was both dose-and time-dependent.Conclusions HSP27-siRNA can inhibit proliferation and induce apoptosis of HCC cancer cell lines.Thus,it may become a method for effective treatment of HCC cancer.