ABSTRACT
OBJECTIVE@#To study the change of laryngeal carcinoma cell apoptosis before and after radiotherapy.@*METHOD@#Living tissue of diseased region obtained from 27 cases of laryngeal carcinoma before and during radiotherapy 10, 30 and 60 Gy were used in this study. With terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL), the spontaneous and radiation-induced apoptotic rate (AR)were examined.@*RESULT@#The AR of living tissue of laryngeal carcinoma before and during radiotherapy 10, 30 and 60 Gy were (21.0 +/- 0.7)%, (60.0 +/- 1.5)%, (42.0 +/- 1.3)%, (25.0 +/- 1.9)%, respectively. The radiation-induced apoptotic rate was significantly higher than that of spontaneous apoptotic rate (P< 0 . 5). In laryngeal carcinoma, spontaneous apoptosis was positively correlated with the radiation-induced apoptosis.@*CONCLUSION@#Detecting of AR of laryngeal carcinoma cells before and during radiotherapy may be helpful to predict the sensitivity of radiotherapy in laryngeal carcinoma patients.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Apoptosis , Radiation Effects , Carcinoma, Squamous Cell , Pathology , Radiotherapy , Dose-Response Relationship, Radiation , In Situ Nick-End Labeling , Laryngeal Neoplasms , Pathology , RadiotherapyABSTRACT
Objective To investigate and evaluate the detection and the variety of histology type constituent of the thyroid malignant tumor before and after universal salt iodization.Methods 1011 clinical pathological data of thyroid malignant tumor confirmed pathologically from 1961 to 2000 was retrospectively analyzed.The detection rate of thyroid malignant tumor,the constituent ratios of each histology type and the changes of age and sex distribution in main types of thyroid malignant tumor were determined.Results The total detection rate of thyroid malignant tumor after universal salt iodization (USI) (0.69%) were obviously increased compared with before universal salt iodization(0.46%,P40 years old) than before USI(≤40 years old).The incidence rates of thyroid malignant tumor in female patients were higher than male patients before and after USI.Conclusion The proportion and average age of thyroid malignant tumor increases after USI.The histological types of thyroid carcinoma have changes after USI:the proportion of PC increases obviously,the proportion of FC decreases accordingly.The average age of thyroid malignant tumor sufferers tends to increase and the peak ages of PC,FC and UC raise after USI.
ABSTRACT
Objective To investigate the effects of advanced glycation endproducts (AGEs) on proliferation of pericytes of bovine retinal capillary vessels and expression of transforming growth factor ?(TGF-?). Methods [WTBZ]The proliferation of pericytes detected by methyl thiazolyl tetrazolium (MTT) colorimetric assay, cellular cycle of pericytes was analyzed by flow cytometry was used to analyze cell, and TGF-? protein expression of pericytes was observed by immunofluorescent staining. Results AGEs inhibited the proliferation of pericytes of bovine retinal capillary vessels, stopped the cellular cycle of pericytes in synthesis phase (S phase), increased the number of apoptotic cells obviously (P