ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of annexin A5 on the apoptosis of laryngeal cancer cells.</p><p><b>METHODS</b>Special siRNAs were used to knock annexinA5 down in Hep-2 cell, and RT-PCR and Western blot were applied to identify the efficacy of RNA interference. The flow cytometry assay was performed to detect the Hep-2 cell apoptosis.</p><p><b>RESULTS</b>RT-PCR analysis showed that the relative mRNA expression of annexin A5 in siRNA group, negative control group, Lipofectamine 2000 group and blank control group were 0.70 ± 0.03, 1.18 ± 0.05, 1.17 ± 0.06 and 1.23 ± 0.07. The relative mRNA expression of annexin A5 in siRNA group was significantly decreased than contrast groups(t = -14.77, t = -13.23, t = -12.99, P < 0.05).In Western blot assay, the trend of protein expression level was consistent with the mRNA expression levels of annexin A5. The relative levels of proteins in siRNA group, negative control group, Lipofectamine 2000 group and blank control group were shown 1.21 ± 0.03, 3.88 ± 0.06, 3.87 ± 0.02 and 3.95 ± 0.08. The relative protein expression of annexin A5 in siRNA group was significantly decreased than contrast groups(t = -70.34, t = -150.62, t = -56.32, P < 0.05). At the same time in flow cytometry the apoptotic rate of siRNA group, negative control group, Lipofectamine 2000 group and blank control group were 4.43% ± 0.12%, 13.67% ± 0.22%, 13.66% ± 0.12% and 13.35% ± 0.13%, the difference between the siRNA group and contrast groups was statistically significant(t = -62.50, t = -14.16, t = -11.47, P < 0.05).So after RNA interference, expression of annexin A5 decreased, and the results in the apoptosis inhibition of Hep-2 cell.</p><p><b>CONCLUSION</b>Annexin A5 promotes apoptosis of Hep-2 cells, and it may be a potential therapeutic target for the laryngeal cancer.</p>
Subject(s)
Humans , Annexin A5 , Genetics , Apoptosis , Genetics , Cell Line, Tumor , Laryngeal Neoplasms , Genetics , Pathology , RNA Interference , RNA, Messenger , Genetics , RNA, Small InterferingABSTRACT
OBJECTIVE@#To investigate the therapeutic effect of obstructive sleep apnea hypopnea syndrome (OSAHS) by modified uvulopalatopharyngoplasty (UPPP).@*METHOD@#Forty-nine cases of OSAHS were treated by modified UPPP, and followed-up on 6 months, 1 year and 2 years after operation and carried out polysomnography (PSG) every time.@*RESULT@#Therapeutic effect was evaluated by standard of Hangzhou (2002) . In 6 months, symptoms of snore, breath holding, headache and lethargy disappeared or reduced obviously, the total effective rate was 100%. In 12 and 24 months, the effective rate was 95.92% and 91.84% respectively.@*CONCLUSION@#This operation can enlarge venting area of palatopharyngeal cavity effectively and keep density of soft palate as well as the normal function of pharyngeal mucous membrane, and can also elevate operative effect and decrease postoperative complications.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Otorhinolaryngologic Surgical Procedures , Methods , Palate , General Surgery , Palate, Soft , General Surgery , Pharynx , General Surgery , Sleep Apnea, Obstructive , General Surgery , Treatment Outcome , Uvula , General SurgeryABSTRACT
OBJECTIVE@#To present the experience of nasal reconstruction with forehead flap.@*METHOD@#nasal reconstruction with forehead flap were applied in eight nasal carcinoma cases after operation and 5 nasal trauma cases with defects.@*RESULT@#These forehead flaps were alive in all patients, all incision healed in I stage, no post operative complications were found. The shapes of nose were satisfactory, there were no recurrence of tumor during 1 to 17 year follow up.@*CONCLUSION@#The method can be clinically applied for its simple procedure, reliable flap's blood supply, high survival rate and satisfied result.