ABSTRACT
Objective:To discuss the influence of different vancomycin administration dosage in elderly patient's through blood concentrations and renal function.Methods:The clinical data about 113 elderly patients treated from January 2016 to December 2018 in our hospital was reviewed. After therapy, the through blood concentrations, administration dosage and renal function changes were detected to analyze their relationship.Results:The peak plasma concentration of 113 elderly patients was significantly higher than that of the young and middle-aged group ( P<0.05). Plasma concentrations in 185 cases were detected, including 35 cases with through blood concentrations<10 mg/L, 71 cases at 10-20 mg/L and 79 cases at >20 mg/L. There were 15 cases with 0.5 g/d of vancomycin, 96 cases at 1.0 g/d, 54 cases at 1.5 g/d and 20 cases at 2.0 g/d. The through blood concentrations among patients with different daily doses were significantly different ( P<0.05); at 1.0g/d, the ratio with through blood concentrations at 10-20 mg/L was the highest; serum creatinine (Cr) and blood urea nitrogen (BUN) contents in patients with through blood concentrations >20 mg/L were higher than those at <10 mg/L and 10-20 mg/L ( P<0.05); before treatment, Cr and BUN contents among groups were not significantly different ( P>0.05); among 113 elderly patients, the results indicated 21 renal function impairment cases and 92 normal renal function cases. The daily doses and through blood concentrations in renal function impairment group were higher than normal group ( P<0.05); BUN contents in renal function impairment group were higher than normal group while endogenous creatinine clearance rate was lower than normal group ( P<0.05); before and after treatment, BUN contents in groups were not significantly different ( P>0.05); after treatment, endogenous creatinine clearance rate for two groups was lower than before ( P<0.05); the results indicated that renal function was negatively correlated with vancomycin administration dosage and positively correlated with through blood concentrations ( P<0.05). Conclusions:During therapy, the renal function shall be closely monitored. According to patient's conditions and through blood concentrations, the vancomycin administration dosage shall be adjusted. In particular, the defined daily doses shall be reduced in renal function impairment patients, which through blood concentrations are kept at 10-20 mg/L.
ABSTRACT
Objective:To study the differentiation capacity of the fibroblast-like cells isolated from human skin dermis into mesenchymal stem cells, and to explore the feasibility to use these cells as alternative cell source of autologus bone marrow mesenchymal stem cells (BMSCs ) for regeneration of tissue inj uries and defects. Methods:Full thickness skin samples were obtained from the abdomen of surgical patients, then digested with dispase and collagenase Ⅰ subsequently. Thereafter, the digested cells were collected and cultured, followed by suspension with serum free medium containing N2,B27,basic fibroblast growth factor (bFGF),and epidermal growth factor (EGF).The skin dermis derived spheroids (SDDSs)were collected and monolayer cultured in serum-containing medium.Finally,the cells were characterized by immunofluorescence staining and differentiation assays.Results:The dermis derived cells proliferated and formed SDDSs in the suspension of serum-free medium. After monolayer cultivation in serum-containing medium, the cells from spheroids were successfully expanded to large number. The cells expressed mesenchymal stem cells markers CD90, CD105 and vimentin. Under osteogenic,chondrogenic and adipogenic differentiation conditions,these cells were differentiated into the alizarin red,safranin O, and oil red O staining positive cells, displayed similar differentiation traits with BMSCs. However,safranin O staining was weaker in the dermis derived cells than BMSCs. Conclusion:A kind of fibroblast-like cells exist in human skin dermis, and have osteocytic, chondrogenic and adipogenic differentiation potentials,demonstrating that these cells will be utilized as a novel cell source for repairing the tissue injury and defect in clinic.
ABSTRACT
Objective To explore whether there is a visual spatial attention span deficit in children with Chi-nese language learning disability and the characteristics of brain electrophysiological dynamic change. Methods Adop-ting event-related potentials ( ERP ) technology and levels of visual spatial attention span with fixed location cuing pa-radigm were used to explore the characteristics of brain electrophysiological dynamic change. Results Based on the behavioral data,the repeated findings of analysis of variance on reaction time showed that both the main effect of the type of subjects[F(1,35)=4. 34,P<0. 05,η2=0. 11],and the main effect of attention span[F(1,35)=138. 75,P<0. 001,η2=0. 79] had statistical significance. The repeated analysis of variance on accuracy showed that the main effect of subjects type[F(1,35)= 4. 99,P<0. 05,η2=0. 13],and the main effect of attention span[F(1,35)=101. 83,P<0. 01,η2=0. 74] were significantly different. Under large and small attention span conditions,the children with Chinese language learning disability showed a longer time of reaction and a lower accuracy rate than the children in the control group. With the electroencephalograph( EEG) data and under large and small attention span conditions,the Chinese learning disability children showed enhanced P1 amplitude and reduced N1 amplitude. Conclusions There is a visual spatial attention span deficit in children with Chinese learning disability which may be caused by a weak prima-ry sensory processing ability and divided attention deficit.