ABSTRACT
Objective The multiple polymerase chain reaction(PCR) technique was adopted to detect AZF microdeletion of Y chromosome in 120 patients with idiopathic oligozoospermia and azoospermia and contemporaneous 60 cases of normal semen rou‐tine examination in the outpatient department .At the same time the peripheral blood chromosome karyotype analysis was performed on these 120 patients .Methods Multiple PCR (Polymerase chain reaction) technique were used to analyze 15 locus of the azoosper‐mia factor (AZF) in the 120 patients with idiopathic azoospermia and oligozoospermia and 60 mormal cases .using the Karyotype a‐nalysis technique to analyze karyotype of the 120 male infertile patients ,at the same time .Results Among 120 cases of male infer‐tility ,no obvious abnormal karyotype was found .23 cases were detected AZFc deletions with the detection rate of 19 .2% ,no mi‐crodeletions at other loci were detected .Conclusion AZFc is the major candidate gene for AZF gene screening in male infertility pa‐tients from this area .The Y chromosome microdeletion is the important cause leading to male infertility .