ABSTRACT
OBJECTIVE@#To explore the relationship between the expression of interferon-induced protein with tetratricopetide repeats 1 (IFIT1) and liver cell apoptosis in the acute stress period after severe burns. @*METHODS@#A total of 25 C57/129 adult mice were randomly divided into the normal control group (0 h) and the groups at 1, 6, 12 or 24 after severe burns (n=5 per group). A model with third degree (20% of the total body surface area) burn injury was established and then liver tissues were taken. IFIT1 expression was examined by Western blot. The expression of caspase-3 and -8 was measured by immunohistochemistry. Liver cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL). @*RESULTS@#After burns, IFIT1 expression was increased at 1 h, which reached the highest level at 6 h followed by a decrease at 12 h, which reached minimum level at 24 h. The differences between groups were significant (P<0.01). The caspase-3 and -8 levels significantly increased after burns in a time-dependent manner (P<0.01). Although at 0 h and 1 h there was no significant increase in liver cell apoptosis, the increase reached significance from 6 h to 24 h (P<0.01). @*CONCLUSION@#The increase in IFIT1 expression after severe burns promotes liver cell apoptosis.
Subject(s)
Animals , Mice , Apoptosis , Blotting, Western , Burns , Metabolism , Carrier Proteins , Metabolism , Caspase 3 , Metabolism , Caspase 8 , Metabolism , Hepatocytes , Cell Biology , In Situ Nick-End Labeling , Liver , Cell Biology , Mice, 129 Strain , Mice, Inbred C57BL , RNA-Binding ProteinsABSTRACT
OBJECTIVE@#To observe the influence of ropivacaine on the proliferation and migration of rat bone marrow mesenchymal stem cells (BMSCs) and provide basis for the clinical application of BMSCs.@*METHODS@#Rat BMSCs were isolated and cultured by adherence method. Surface markers of BMSCs were examined by flow cytometry. Multipotent differentiation of BMSCs was detected by induced adipogenesis, osteogenesis and muscular differentiation. Proliferation of BMSCs was examined by CCK-8 and Brdu incorporation after ropivacaine treatment at different concentrations. Migration of BMSCs was tested by cell scratch assay and Millicell experiment.@*RESULTS@#Cultured cells had representative appearance and surface markers of BMSC, and they had potential multiple differentiation. Ropivacaine treatment at 50 and 100 μmol/L significantly reduced the proliferation rate of BMSCs and Brdu incorporation rate. There was significant difference compared with the control group (P<0.05). Cellular scratch assay and migration experiment indicated that ropivacaine significantly reduced the migration of BMSCs. There was significant difference compared with the control group (P<0.05). All these mentioned effects of ropivacaine on BMSCs were dose-dependent. There was significant difference between groups (P<0.05).@*CONCLUSION@#Ropivacaine can significantly reduce the proliferation and migration of rat BMSCs, suggesting that the influence of local anesthetics on BMSCs has to be taken into account when BMSCs are used in clinical practice.
Subject(s)
Animals , Rats , Amides , Pharmacology , Bone Marrow Cells , Cell Differentiation , Cell Movement , Cell Proliferation , Cells, Cultured , Flow Cytometry , Mesenchymal Stem Cells , Cell Biology , RopivacaineABSTRACT
Objective:To evaluate the effect of ketamine on the expression level of hepatic stress protein IFIT1 and JAB1 during the early stage of burns in mice, and observe the location of IFIT1 and JAB1 in hepatic cells. Methods:15 C57/129 male mice were divided randomly into three groups(n=5): normal control,burns,burns+ketamine. Burns group and burns+ketamine group were inflicted with 15%~20% TBSA full thickness burn injury,and burns+ketamine group received an intramuscular injection of 10 mg/kg ketamine 15 min after burns. At 4 h after burns,hepatic tissue was taken from mice,and the levels of hepatic I- FIT1 and JAB1 were detected by western blot. Normal control hepatic pathological section was taken; then cell location of I- FIT1 and JAB1 was detected by immunohistochemistry. Results:In burns group, the expression level of hepatic IFIT1 signifi- cantly increased,while that of JAB1 decreased as compared with normal control(P