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Objective To investigate the potential role of Lycium bararum polysaccharide (LBP) with or without interferon -inducible protein 10 ( CXCL10) in inducing dendritic cells ( DC) functional maturation by monitoring the alteration of cytokines for inducing DC maturation in peripheral blood and by detecting the expression of S-100 protein in tumor tissue, thus to reveal its mechanism of inhibiting experimental liver cancer. Methods H22 bearing mice model was established. The mice were randomized into model group, LBP group (50 mg/kg, ig), CXCL10 (right axillary subcutaneous injection of 15 μg/kg), LBP + CXCL10 group (LBP 50 mg/kg, ig, and right axillary subcutaneous injection of CXCL10 15 μg/kg), 5- fluorouracil (5FU) group ( intraperitoneal injection of 12mg/kg) , 12 mice in each group. The mice were administered the corresponding medicine once a day. After treatment for 2 continuous weeks, blood was sampled from infraorbital vein, and the tumor mass, spleen, thymus were extracted for the calculation of anti-tumor rate, thymus index and spleen index separately . The mRNA expression levels of interleukin 12 (IL-12) and tumor necrosis factor-α (TNF-α) in peripheral blood were detected by fluorescence quantitative PCR, the expression of S-100 protein in tumor tissues was detected by immunohistochemical assay. Results Compared with the model group, tumor growth in LBP group and LBP+CXCL10 group was obviously inhibited, and tumor-inhibitory rate was 55.90%, 50.91%, respectively. Meanwhile, the mRNA expression level of IL-12 was 2.94 folds higher in LBP group and 3.39 folds higher in LBP + CXCL10 group, and TNF-α mRNA expression level was 1.55 folds higher in LBP group and 4.74 folds higher in LBP+CXCL10 group than the model group, the differences being statistical significant ( P<0.05 or P<0.01). Results of immunohistochemical assay showed that S-100+DC number in LBP group and LBP+CXCL10 group was larger than that in the model group (P<0.05 ). Conclusion LBP and LBP+CXCL10 exert significant effect on inhibiting experimental liver cancer. The mechanism may be related with inducing the secretion of IL-12 and TNF-α, which plays a key role in inducing DC maturation, and with the increase of the number of DC in tumor microenvironment.
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Purpose:To explore the value of predicting the axillary lymph node(ALN) metastases statu s by sentinel lymph node biopsy(SLNB) with methylene blue subcantaneously inject ion in the breast cancer patient and to provide the scientific basis for selecti ve axillary lymph node dissection(ALND) in breast cancer.Methods :1% methylene blue 2ml was subcantaneously injected at 4 sites of the skin over the tumor in each of 64 patients with breast cancer. Most of these were staged as T 1-2 N 0M 0, and a few were T 3N 0M 0. All patients underwent a S LNB 5 minutes after injection, followed by various ways of radical operation. Pa thohistological examination was assessed in all of SLN which included a frozen s ection intra-operation and a paraffin section after operation, and all other no n-SLN had a paraffin section.Results:The successful detection rate of SLN was 85.9%(55/64), and the accuracy of predi cting ALN metastases was 96.4%(53/55). The sensitivity was 90.9%(20/22), the sp ecificity was 100%(33/33), and the false-negative rate was 9.1%(2/22). The pred ictive value of a positive test and of a negative test were respectively 100%(22 /22) and 94.3%(33/35). In the resected SLN and non-SLN the metastases rates wer e respectively 29.4% and 8.3%(? 2=41.493, P
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PURPOSE To discuss induction and relation of synchronous multiple primary cancer to adenoma of large intestines. METHODS Continuous observation of resectable and pathologically proved cancer of large intestines of 166 cases. RESULTS We discovered 8 cases (4. 8%) of synchronous multiple primary cancer of large interstines. 43 cases of single primary cancer of concomitant adenoma (27. 7%). The 4 cases of synchronous cancer had merged adenoma (50%). The 4 cases of synchronous cancer had concomitant adenoma of 14 sites. Of these 2 cases had neoplastic change in 3 sites. The rate of neoplastic change was 50% concomitant adenoma. In the whole group, of the 18 cancer sites, 8 nidi were less than 3cm. CONCLUSION Synchronous cancer of large intestines is not rare, and is related to malignant change of adenomas. complete colonoscopy is an important means to improve diagnostic rate.