ABSTRACT
ObjectiveTo find out the problems and deficiencies in colposcopy through the quality control of colposcopy in 19 hospitals in Zibo City， so as to put forward constructive opinions and suggestion to improve the accuracy and standardization of colposcopy by the doctors in local hospitals. MethodsA total of 21 hospitals with colposcopy examination in Zibo City were selected as the research object. The quality control standards were formulated and the case data in the early stage were evaluated. Two hospitals had less than 10 cases completed each year and no on-site quality control.A total of 19 hospitals had on-site quality control with the operation process of colposcopy instrument on site， the configuration of colposcopy， the proportion of colposcopy examination reagents used in the department of pathology， and the operation period of colposcopy examination， as well as the on-site observation report. The qualified rate of each hospital were counted and analyzed. ResultsThe quality control results of 19 hospitals showed that the qualifications and the clinical practice of obstetrics and gynecology for more than 3 years reached 100.00%. Those who complete more than 100 new diagnosis cases each year and those who have been trained for more than 3 months are gradually increasing. The pathological specimens met the needs and the positive predictive value of high-grade squamous intraepithelial lesion was significantly improved in the quality evaluation indicators of colposcopy （all P<0.05）.In the basic elements of the colposcopy report， there were statistically significant differences in 5 aspects：adequacy of the examination， the description of specific images， the interpretation of key images， the diagnosis of the colposcopy， and the post-examination processing through 2 colposcopy quality control comparisons （all P<0.05）. ConclusionThe overall level of colposcopy in Zibo City has been significantly improved through the comparison of the quality control results of two colposcopy. We can improve the quality of colposcopy effectively through regular quality control.
ABSTRACT
Objective:To establish an air-pouch bladder cancer (APBCa) model and investigate whether it could be a new animal model to evaluate the efficacy of intravesical therapy through chemotherapeutics and BCG instillation.Methods:Filtered sterile air was injected subcutaneously into the backs of BALB/c Nude mice to create a 2.5 cm×3.5 cm air pouch. After 24 hours, human bladder cancer cells EJ were seeded on the inner face of the pouch wall to establish APBCa of human cancer (H-APBCa). Gemcitabine instillation was used to investigate whether chemotherapy could inhibit tumor growth in the H-APBCa model, and Tunel staining was used to verify the apoptosis of tumor cells 20-day treatment. Filtered sterile air was injected subcutaneously into the backs of C57BL/6 mice to create a 2.5 cm×3.5 cm air pouch. After 24 hours, mice bladder cancer cells MB49 were seeded on the inner face of the pouch wall to establish APBCa with intact immunity (I-APBCa). BCG instillation was used to investigate whether BCG could inhibit tumor growth in the APBCa model. Immunofluorescence was used to verify the infiltration of immune cells after 20-day treatment.Results:H-APBCa and I-APBCa mice models could be established by immune deficiency and intact mice. At day 20, chemotherapeutic instillation therapy could inhibit tumor growth (781.02±241.02 vs. 1213.88±214.02 mm 3, P<0.05) by inducing tumor cell apoptosis with statistically significant differences (77.33±4.63 vs. 14.67±2.60, P<0.05). BCG instillation was able to inhibit tumor growth (645.02±156.63 vs. 948.84±221.76, P<0.05) by increasing CD80 + macrophage (49.67±7.57 vs. 16.33±5.69, P<0.05) and T cells in the tumor with statistically significant differences (18.00±3.46 vs. 4.67±1.45, P<0.05). Conclusions:APBCa model could evaluate the efficacy of drug instillation and was expected to be a new animal model for studying drug for intravesical therapy.