ABSTRACT
Novel and efficient therapeutic modalities are urgently needed for cancer patients .We focused on biological therapies including oncolytic virotherapy and immune therapy against cancer .In the study of oncolytic virotherapy , we have identified the crosstalk between virus-induced mitophagy and retinoic acid inducible gene I (RIG-I)-like receptors signaling.Importantly, we find that cell necrosis rather than apoptosis ( as supposed by previous studies ) contributes dominantly to oncolysis of measles virus Edmon-strain in cancer.Furthermore, we have clarified the role of mitophagy in switching cell death from apoptosis to necrosis .These novel findings are important for further improvement of oncolyticvirotherapy .5′-Triphosphate RNA ( ppp-RNA) is a specific ligand of the pat-tern recognition receptor retinoic acid-inducible gene I ( RIG-I) .By combining immune activation and specific gene silencing , we have designed three anticancer ppp-siRNAs targeting TGF-β1 , GLS1 and VEGF , respectively , and we find that these ppp-siRNAs possess potent antitumor efficacies both in vitro and in vivo .In addition , we have also utilized nanomaterials to treat cancers .Next, we want and try to block the immune escape of cancer by targeting immune checkpoints .
ABSTRACT
AIM: This study is based on the result of the study in HL and ALCL employing gene chip technique, in which writer found that there was distinctly different expression of caspase-4 between HL and ALCL cell lines at the level of mRNA. From the point of view, we try to identify at the level of protein whether there is different expression of this gene in HL and ALCL tissues as well. METHODS: HE staining, the monoclonal antibodies CD30 (BerH2), CD15 (C3D-1), CD20 (L26) and CD45RO (UCHL1) were used for selecting the cases of HL and ALCL. Specific high affinitive anti-caspase-4 polyclonal antibody was used by immunohistochemical staining to analyze the expression of caspase-4 in 18 cases of HL and 15 cases of ALCL. RESULTS: The expression of caspase-4 demonstrated a strong positive staining in all ALCL cases (15/15,100%), whereas negative in 16 HL cases (88 8%), while other two cases were weakly stained (11 2%), showing a distinct difference (P