ABSTRACT
Objective:To optimize the extraction process of Xiaoyan granules. Methods:The extraction process was optimized by orthogonal test with water volume, extraction times and extraction duration as the influencing factors. The dry extract rate and esculetin content were used as the evaluation indices. The dry extract rate was determined by drying method, and esculetin was determined by HPLC. Results:The optimal extraction conditions were as follows:water was 8 times of the medicinal materials, the decoction duration was 1. 5h with 3 extraction times. Conclusion:The optimized process of Xiaoyan granules is feasible and convenient,and the granules are qualified and stable.
ABSTRACT
Objective:To investigate the component changes in compound granatum berberine capsules after the radioactive ray ir-radiation sterilization. Methods: The chromatographic fingerprints were obtained on a Diamonsil C18 analytical column ( 250 mm × 4. 6 mm,0. 5 μm) with solvent system composed of acetonitrile-0. 033 mol·L-1 potassium dihydrogen phosphate, the flow rate was 1. 0 ml·min-1 and the detection wavelength was set at 265nm, the column temperature was maintained at 30℃, and the injection vol-ume was 10 μl. Results:Totally 10 co-possessing peaks were selected as the fingerprint peaks for compound granatum berberine cap-sules, and the fingerprints exhibited promising separation for each peak and revealed the fingerprint information according to the techni-cal requirements of fingerprints for Chinese traditional medicines. Most components were with different degrees of reduction, and some peaks even lost after the irradiation. Conclusion:The main ingredients show no statistically significant change after irradiation ( P>0. 05), suggesting the sterilization method is suitable for the preparation.
ABSTRACT
Objective:To compare the content of icariin in Rujiling granules before and after the ray irradiation. Methods: The column was Thermo ODS-2 HYPERSIL (250 mm × 4. 6 mm,5 μm) with the temperature of 30℃, and the mobile phase was acetoni-trile-0. 033 mol·L-1 potassium dihydrogen phosphate(30∶70) with the flow rate of 1. 0 ml·min-1. The detection wavelength was 265nm. Results:There was a good linear relationship within the range of 3. 44-34. 40μg·ml-1 for icariin (r=0. 999 7, n=6). The average recovery was 98. 18% with RSD of 0. 32%(n=9). Conclusion:The content change of icariin in Rujiling granules before and after the ray irradiation is not statistically significant, which illustrates that the conventional dose irradiation has little effect on icariin.
ABSTRACT
This paper was aimed to study the quantitation method of bitterness intensity of bitter drug, and to ex-plore the mutual relations between the bitterness intensity and the concentration of bitter drugs solution. The mod-el drug of berberine hydrochloride, which was made into a series of solutions of different concentrations, was ap-plied. Twenty volunteers, who were screened, trained and qualified, tasted the different concentration of aqueous solution, with the purpose of detecting the rank bitterness intensity. After analyzing the features of the data and testing the logarithm model and Weibull curve model, as well as nonlinear fitting, we finally set up the appropri-ate relationship curve between the concentration and the bitterness intensity. There is a good curve positive corre-lation between the bitterness intensity and the concentration, which is consistent with the Weibull curve model (R2= 0.9973, RMSE = 0.0976, RMSECV = 0.1453). The fitting degree of the model established in this paper is high, which provides a reference for quantitation and forecast of the drug bitterness.
ABSTRACT
Taste-masking effect of pharmaceutical formulation is important for the pharmacy worker to carry out study on taste-masking of pharmaceutical formulation. This study discussed the feasibility of electronic tongue in the evaluation of taste-masking effect. The berberine hydrochloride was used as model drug of bitterness. Sodium cycla-mate, 2,4-Dihydroxybenzoic acid and sodium cyclamate were used as bitterness inhibitors. Through detection of elec-tronic tongue, results from principal component analysis (PCA), and indexes such as distance of bitterness (D), dis-tance of bitterness reduction, inhibition rate (Ir), the determination was given on whether results from the bitterness inhibition of berberine hydrochloride and taste results from volunteers were the same. In addition, sodium cyclamate, which was the best bitterness inhibition, was made into different concentrations in order to optimize its using dosage. The data analysis results showed that in the berberine hydrochloride solution with the concentration of 0.005 mg/mL, the ranking order of the bitterness-masking efficiency was sodium cyclamate > 2,4-Dihydroxybenzoic acid > sodium acetate anhydrous. Along with the increasing concentration of sodium cyclamate, the taste-masking effect is better. However, after the concentration level of 0.2%, the change on taste-masking effect was relatively small. In the prac-tical work, the concentration of sodium cyclamate can be selected at the level of 0.2%. This experiment effect was the same as the human taste results. This study showed that the electronic tongue may be a useful tool in the evalua-tion of taste-masking efficiency to some extent.