ABSTRACT
Objective To explore the feasibility of removal of affected segments via laparoscopic surgery in patients with mild colorectal perforation. Methods The clinical data on sixty-one patients with colorectal perfo-ration who had been treated in our hospital between June 2006 and June 2016 were retrospectively analyzed. The patients were divided into an open surgery group(group OS,n=38)and a laparoscopic surgery group(group LS, n = 23). All the procedure-related data were collected and analyzed. P < 0.05 was considered statistically signifi-cant in this study. Results The volume of blood loss was smaller in group LS than in group OS[(100 ± 165)mL vs(200 ± 205)mL,P=0.032],hospital stay was shorter in group LS than in group OS[(7 ± 5.21)d vs.(10 ± 9.2)d ,P = 0.009],and rate of wound infection was lower in group LS than in group OS(4/23 vs. 13/38,P =0.039),while surgical duration was longer(213.7 ± 65.7 min vs. 173.5 ± 47.2 min,P = 0.047). There were no significant differences between the two groups in intraoperative blood transfusion[(0.8 ± 1.1)U vs.(0.4 ± 0.7)U , P=0.270],postoperative ICU stay[(2 ± 0.9)d vs.(2 ± 0.7)d,P=0.009],rate of postoperative complications 25/38 vs. 14/23),and hospitalization costs[(31 758 ± 29 283)yuan vs.(36 243 ± 27 584)yuan,P = 0.673]. Conclusions Laparoscopic colectomy is safe and feasible in the treatment of mild colorectal perforation.
ABSTRACT
Objective To investigate the alternation of insulin -like growth factor -Ⅱ( IGF-Ⅱ) gene promoter P4 methylation status in hepatocellular carcinoma ( HCC) and explore its relationship with expression of P4 mRNA levels.Methods Liver specimens of 43 patients with HCC and normal liver specimens of 9 control patients were collected in operation .Tissue DNA and total RNA were extracted from these specimens .IGF-Ⅱ P4 methylation status and P4 mRNA expression levels were detected .Results (1)The incidence of IGF -ⅡP4 methyl-ation in HCC group was significantly lower than that in normal liver specimens (16.28%vs 88.89%,χ2 =19.12,P<0.01).(2)The expression level of IGF -ⅡP4 mRNA in HCC group was significantly higher than that that in normal liver specimens[(0.96 ±0.74) vs (0.25 ±0.19),t=5.48,P<0.01].(3)In HCC group,the IGF-Ⅱ P4 mRNA expression level with hypomethylation gene was significantly higher than that without hypomethylation gene [(1.18 ± 0.76) vs (0.32 ±0.27),t=5.28,P<0.01].Conclusion The hypomethylation alternation of IGF -Ⅱ P4 gene promoter which is accomplished by up -regulate P4 mRNA expression has a close relationship with HCC .
ABSTRACT
Objective To investigate the protective role of Ulinastatin UTI on gut barrier of septic rats. Methods Twenty-two SD rats were divided into three groups: sham laparatomy(S), cecal ligation and punture(CLP), and CLP plus UTI. Septic rat model was estabilished through CLP method. Fluorescence spectrometry was used to measure FITC-dextran concentration from bowel lumen to portal vein. Ultrastructure of intestinal mucosa was observed under transmission eletron microscope (TEM). Results Twenty-one hrs after CLP septic symptoms in CLP plus UTI group were milder than those in CLP group. Portal concentration of FITC-dextran in CLP group was higher than that of S group[S (1.22?0.21) ?g/ml vs. CLP (2.51?0.56) ?g/ml, P