ABSTRACT
Aim To test the skin healing and repairing efficacy and the mechanism of Hibiscus rosa-sinensis L bud extract by using the animal models. Methods KM mice were randomly divided into three groups:the model group, the positive control group, and the n-bu-tyl alcohol extract ( HrBN) group. Using the boils and carbuncles model, the healing condition of all the animals were observed. KM mice were kept in the SPF condition room and divided into five groups: the model group, the positive control group, and the low, middle, high dose groups. Using the full-thickness loss model, the repairing results of all the mice were ob-served. Through the antimicrobial test, the results of MIC and inhibition zone were obtained. The carbon clearance test was used to collect the blood at the time 5min and 15min, and get the liver and spleen, and the results of K andαwere obtained. Results In vivo ex-periments showed there was significant difference be-tween groups;the HrBN extract had the outstanding ef-ficacy in healing and repairing skin boils and full-thickness loss models. It had higher recovery rate than other ethanol extract, such as ethyl acetate extract and chloroform extract. In vitro experiments showed that the HrBN extract, ethyl acetate extract ( HrBE) ,AB-8 macroporous resin 30% alcohol part and 60% alcohol part had obvious antimicrobial efficacy. The carbon clearance test showed HrBN had a good effect in im-proving immune function, and it can increase the K and α. Conclusion HrBN in animal models exerts good skin healing and repairing efficacy, which might be related to its antibacterial activity and immunologic enhancement function.
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether bone marrow stromal cell line QXMSC1 (H-2(d)) engineered to secrete IL-3 (QXMSC1 IL-3) can improve the hematopoiesis post-allogeneic bone marrow transplantation (BMT) in mice.</p><p><b>METHODS</b>The stromal cell line QXMSC1 IL-3 was established by transfecting QXMSC1 (H-2(d)) cell with a recombined retrovirus vector PL3SN containing mice IL-3 gene cDNA. Lethally irradiated mice C57BL/6 (H-2(b)) were transplanted with T cell depleted allogeneic bone marrow (BALB/c, H-2(d), 1 x 10(7)/mice) and QXMSC1 IL-3 cells (5 x 10(5)/mice). The numbers of RBC and WBC in peripheral blood were counted 20 and 40 days after bone marrow transplantation. The marrow nucleated cells, CFU-S, CFU-GM, CFU-E and CFU-GEMM yields were measured in recipient mice.</p><p><b>RESULT</b>QXMSC1 IL-3 cells could stably secrete IL-3 and increase the peripheral RBC and WBC counts as well as the number of marrow nucleated cells and CFU-GM, CFU-E, CFU-GEMM yields.</p><p><b>CONCLUSION</b>Cotransplantation of QXMSC1 IL-3 cells with T cell depleted marrow grafts improve hematopoiesis post allogeneic BMT in mice.</p>
Subject(s)
Animals , Female , Mice , Bone Marrow Transplantation , Erythrocytes , Cell Biology , Hematopoiesis , Genetics , Physiology , Interleukin-3 , Genetics , Leukocytes , Cell Biology , Mice, Inbred BALB C , Mice, Inbred C57BL , Stromal Cells , Metabolism , Transplantation , Time Factors , TransfectionABSTRACT
Adenosine is an endogenous purine uncleoside released by cells as part of the normal metablic mechinary. During inflammation, massive ATP degradation increases the local adenosine concentration to the micromolar range, at the range, adenosine extert potent antiinflammatory rale. In vivo or in vitro experiments, the addition exogenious adenosine, its analogues and inhibition of its degradation attenuate injury of animal models of inflammation. Antiinflammatory mechinasms involve in;(1)Adenosine inhibites neutrophil function in vitro, including chemotaxiz, adhesion, phogocytosis, and oxygen radical generation. (2) Decrease the expression of collagenase, and reduce the amount of collagenase. (3)Blocking neutrophil adhesion to the endothelium mediated by L-selectin and ftrEategrin. (4)inhibition activated humanmonocytes and macrophage secreting cytokins. (5) Adenosine enhances IL-10 secretion by human monocytes. (6)inhibition of immune response and so on. Relation with nonsteroidal antiinflammatory drugs (NSALDs) includes:(1)MTX and SASP inhibit the activity of 5-aminoimidazole-4-carboxam-ide ribonucleotide (AICAR) transformylase resulting in increased local adenosine concentraton. (2) Azathioprine and its potential metabolite inhibits adenosine kinase, diminish AMP formation by adenosine. In conclusion, the existance of a novel class of antiinflammatory agents, affects adenosine metabolism and may be a useful antiinflammatory drug as well.