ABSTRACT
PURPOSE: The present study was undertaken to determine the 24 - hour pattern of intraocular pressure (IOP), and to reveal the effect of unilateral decentralization on circardian rhythm of IOP in eyes of rats. METHODS: Nine male Sprague - Dawley rats were exposed to a 12 - hour light / dark cycle for 3 weeks. 24-hours IOP pattern was measured at six time points (8, 12, 16, 20, 24, and 4 circardian time) by Tonopen XL tonometer in awake state. After unilateral sympathectomy (cervical ganglionectomy), IOP measurements were performed at the same time points on post-operation 1, 2, 3, and 4 weeks. RESULTS: 24 - hour IOP pattern of rats showed a rhythmic pattern that appeared to be sinusoidal with 12 - hour light / dark cycle. IOPs were low during the light phase and high during dark phase. After unilateral sympathectomy, the circardian rhythm of IOP was maintained in control eyes. But in sympathecotmized eyes, the circardian thythm of IOP was loss. CONCLUSIONS: These results show that sympathetic nervous systems contribute to the circardian rhythm of IOP in rats.
Subject(s)
Animals , Humans , Male , Rats , Eye , Intraocular Pressure , Light , Politics , Sympathectomy , Sympathetic Nervous SystemABSTRACT
Expander procedure is a newly developed extraocular muscle weakening technique which has several advantages such as good control of resection margin, easy reoperation, rare postoperative muscular paresis. For the study of usability of Gore-Tex(R) as a muscle expander, fifteen rabbits received expander procedure with Gore-Tex(R) in right eye and silicone retinal band in left eye. At postoperative 1, 3, 5, 7, 12 weeks, histopathologic examination was performed. There was no significant difference in inflammatory reaction in both groups. Inflammatory reaction decreased with time in both groups. In Gore-Tex(R) expander group, muscle regeneration from cut end was observed at the 3rd postoperative week and fibroblast ingrowth into the expander was observed at the 5th postoperative week. These findings were more prominent with time. Adhesion of expander to underlying sclera was not observed in both groups. It is concluded that expander procedure using Gore-Tex(R) will be a new and useful surgical method for the extraocular muscle weakening procedure.
Subject(s)
Rabbits , Fibroblasts , Paresis , Regeneration , Reoperation , Retinaldehyde , Sclera , SiliconesABSTRACT
To investigate the applicability of the Bartha strain of pseudorabies virus(PRV-Ba) as a neurotracer in the visual system, we performed immunohistochemical study of the rat brain after intravitreal injection of the PRV-Ba. Cholera toxin-horseradish peroxidase(CT-HRP), a well known neurotracer, was also injected into the vitreous cavity for comparison. After given survival time the brain was removed and processed immunohistochemical stain for PRV-Ba and neurohistochemical stain for CT-HRP. The CT-HRP positive reactions were observed almost contralaterally in optic chiasm, optic tract, suprachiasmatic nucleus(SCN), olivary pretectal nucleus(OPT), all three subdivision of lateral geniculate body, superficial gray layer and optic layer of superior colliculus and medial, lateral, dorsal terminal nucleus of accessory optic tract. PRV-Ba immunopositive reaction was observed in SCN and OPT, but not in nerve fiber system and dorsal lateral geniculate body. Also PRV-Ba positive cells were distributed mainly in intermediate layer and weakly distributed in superficial layer of the superior colliculus. This study suggests that PRV-Ba is not a useful neurotracer in studying the visual system of the rat.
Subject(s)
Animals , Rats , Brain , Cholera , Geniculate Bodies , Herpesvirus 1, Suid , Intravitreal Injections , Nerve Fibers , Optic Chiasm , Pseudorabies , Superior Colliculi , Visual PathwaysABSTRACT
A potent antiproliferative agent, Mitomcin-C, has been known to improve the surgical outcom of glaucomatous eyes with poor prognosis after filtering surgery. Twenty one eyes of 21 patients underwent trabeculectomy with Mitomyc-C. Overall success rate is 81%: 2 of 3 eyes with glaucoma after unsuccessful filtering surgery, 3 of 4 eyes with neovascular glaucoma, 2 of 3 eyes with secondary glaucoma, 1 of 2 eyes with aphakic glaucoma and all of 9 eyes with advanced glaucoma. Postoperative complications were prolonged conjuntival wound leakage in two eyes, conjuntibval wound leakage and corneo-lenticular touch in one eye, choroidal detachment in one eye, progression of cataract in one eye, hyphema in 3 eyes.
Subject(s)
Humans , Cataract , Choroid , Filtering Surgery , Glaucoma , Glaucoma, Neovascular , Hyphema , Mitomycin , Postoperative Complications , Prognosis , Trabeculectomy , Wounds and InjuriesABSTRACT
A potent antiproliferative agent, Mitomcin-C, has been known to improve the surgical outcom of glaucomatous eyes with poor prognosis after filtering surgery. Twenty one eyes of 21 patients underwent trabeculectomy with Mitomyc-C. Overall success rate is 81%: 2 of 3 eyes with glaucoma after unsuccessful filtering surgery, 3 of 4 eyes with neovascular glaucoma, 2 of 3 eyes with secondary glaucoma, 1 of 2 eyes with aphakic glaucoma and all of 9 eyes with advanced glaucoma. Postoperative complications were prolonged conjuntival wound leakage in two eyes, conjuntibval wound leakage and corneo-lenticular touch in one eye, choroidal detachment in one eye, progression of cataract in one eye, hyphema in 3 eyes.
Subject(s)
Humans , Cataract , Choroid , Filtering Surgery , Glaucoma , Glaucoma, Neovascular , Hyphema , Mitomycin , Postoperative Complications , Prognosis , Trabeculectomy , Wounds and InjuriesABSTRACT
Using immunohistochemical technique, we observed time-sequentially to T and B lymphocyte in the ciliary body of the endotoxin induced uveitis in rat. The primary antibodies for immunohistochemical stain of the lymphocyte were IgM heavy chain, IgG heavy chain, T suppressor/cytotoxic (CD8) and T helper/macrophage (CD4) was performed in the frozen section. The intensity of immunohistochemical reaction for surface IgM positive cells increased from 12hours and reached most strong intensities at 24hours after endotoxin injection and then decreased rapidly, but the intensity for surface IgG positive cells increased from 48hours and sustained 96hours. There were no significant changes of intensity of immunohistochemical reaction for T cells except slightly increased T suppressor/cytotoxic cells (CD8).
Subject(s)
Animals , Rats , Antibodies , Ciliary Body , Frozen Sections , Immunoglobulin G , Immunoglobulin M , Lymphocytes , T-Lymphocytes , UveitisABSTRACT
In order to explore the existence and distribution of phospholipase (PLC) isozymes in the rat retina, immunohistochemical staining was applied using monoclonal antibodies against PLC isozymes (PLC beta; K92, PLC gamma; D7, F7, PLC delta; R32, S11). For immunohistochemical detection, avidin-biotin peroxidase complex (ABC) method was performed on frozed tissue sections of rat retina. Our study showed that PLC isozymes have particular distributional patterns in the retina. Namely, PLC beta is broadly distributed in the outer and inner segments of photoreceptor cell layer, nuclear layer and ganglion cell layer. PLC gamma is mainly appeared in the nerve fiber layer, ganglion cell layer and inner nuclear layer. PLC delta is confined only in the ganglion cell layer. These results clearly demonstrate the PLC isozymes may have their own role in the transduction of light pathway in the retina. However, further studies will be required to verify theirs precise role in the photoreception.
Subject(s)
Animals , Rats , Antibodies, Monoclonal , Ganglion Cysts , Immunohistochemistry , Isoenzymes , Nerve Fibers , Peroxidase , Phospholipase C beta , Phospholipases , Photoreceptor Cells , Retina , Type C PhospholipasesABSTRACT
In order to explore the existence and distribution of phospholipase (PLC) isozymes in the rat retina, immunohistochemical staining was applied using monoclonal antibodies against PLC isozymes (PLC beta; K92, PLC gamma; D7, F7, PLC delta; R32, S11). For immunohistochemical detection, avidin-biotin peroxidase complex (ABC) method was performed on frozed tissue sections of rat retina. Our study showed that PLC isozymes have particular distributional patterns in the retina. Namely, PLC beta is broadly distributed in the outer and inner segments of photoreceptor cell layer, nuclear layer and ganglion cell layer. PLC gamma is mainly appeared in the nerve fiber layer, ganglion cell layer and inner nuclear layer. PLC delta is confined only in the ganglion cell layer. These results clearly demonstrate the PLC isozymes may have their own role in the transduction of light pathway in the retina. However, further studies will be required to verify theirs precise role in the photoreception.