ABSTRACT
PURPOSE: Human herpesviruses have been associated with the etiology of several human cancers. The role of these viruses in carcinogenesis has not yet been clarified. This study focused on identifying and characterizing the transforming potential of cloned DNA fragments from human cytomegalovirus (HCMV). MATERIALS AND METHODS: Multiple DNA fragments of HCMV were applied to cells for transformation. Morphological transforming region II (mtrII) of HCMV strain Towne has been identified to a 3.0kb XbaI-BamHI DNA fragment which was retained in transformed cells. The transforming activity was induced by a 980 bp BaII-Xho I subfragment (pBS980) containing both promoter/ regulatory elements as well as three open reading frames (ORFs), i.e., 79ORF, 83ORF, and 34ORF. The ORFs have been evaluated for transforming potential in NIH3T3 cells. RESULTS: MtrII (pBS980) has BglII restriction enzyme site which divides into two subfragments, pBS440 and pBS540, the latter has whole 83ORF, 34ORF, and fragment of 79ORF, the former has only fragment of 79ORF. Among three ORFs, 83ORF and 34ORF were not functional in transformation, because in pBS540 these ORFs were not truncated. CONCLUSION: The 79ORF (79-aa transforming peptide) has allowed a better approach to determine the role of HCMV in human carcinogenesis.
Subject(s)
Animals , Humans , Carcinogenesis , Clone Cells , Cytomegalovirus , DNA , Ecthyma, Contagious , Herpesviridae , Open Reading FramesABSTRACT
No abstract available.
Subject(s)
Colon , Colonic Neoplasms , Dimenhydrinate , Oncogenes , Rats, WistarABSTRACT
A case of high jejunal obstruction due to stricture is reported. It is a rare form of late sequelae of neonatal necrotizing enterocolitis(NEC). Barium contrast study of the small bowel is recommended for earlier diagnosis and treatment before discharge from the hospital, even is an asympto-matic patient.
Subject(s)
Child, Preschool , Humans , Male , Constriction, Pathologic , Enterocolitis, Pseudomembranous/complications , Intestinal Obstruction/etiology , Jejunal Diseases/etiologyABSTRACT
The primary hyperparathyroidism, accompanied with a wide scope of bone resorption in every bone matrix of the body, cyst formation, deformity and pathologic fracture, is a generalized disease which causes atrophy and weakness of skeletal muscle and a disturbance of the digestive system as well as renal function. The primary cause of this disease is an adenoma and a hyperplasia of the parathyroid gland. However, all the progress of the disease can be stopped through surgical excision. The authors medically confirmed the diagnoses through typical radiologic findings, electrolyte and enzyme level of serum and urine and by carotid arteriography on four patients(a 41 year ald male, and 28, 35 and 36 year old females) who had complained of generalized weakness, pain and motion limitation. The authors obtained the following results after surgical excision and continuous follow-up study with medication for 7 years, 3 years, 17 months and 13 months each: 1. Of the four cases, 3 cases were female patients and 1 case was a male patient which showed females outnumbered males 3 times in our study. 2. By age distribution, most cases fell in middle age. 3. All the cases were chief cell type of adenoma. 4. It is conjectured that the symptoms has nothing to do with age or history. 5. In all the cases, serun calsium showed a marked decrease immediately after the surgery but recovered to normal limits within 2 months. 6. In all the cases, serum phosphorus showed an irregular increase immediately after the surgery but also stavilized gradually to normal limits within 2 to 3 months. 7. In all the cases, serum alkaline phosphatase was stable for 3 months after the surgery, then gradually decreased. 8. According to the X-rays, subperiosteal resorption of phalangeal bones almost completely disappeared in 3 months. Cyst and brown tumor disappeared in 6~12 months. Granular mottling of the skull almost completely disappeared in 6 months. Intra articular calcification in 2 cases almost completely disappeared in 6 months, 3 cases which showed loss of lamina dura developed edentia during treatment. 9. In 4th case, small multiple cysts and several small brown tumors of the pelvis healed well, but several lager ones were still unhealed after 10 years of treatment. In first case, the cyst in proximal shaft of the left femur is reduced in size but still evident after 7years of treatment. This case needs further study and observation.