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1.
Journal of Zhejiang University. Science. B ; (12): 525-534, 2018.
Article in English | WPRIM | ID: wpr-772769

ABSTRACT

OBJECTIVE@#The aim of the current study was to evaluate the effect of ultraviolet (UV) photofunctionalization of dental titanium implants with exposure to the oral cavity on osseointegration in an animal model.@*METHODS@#Forty-eight titanium implants (Camlog Conelog 4.3 mmx9.0 mm) were placed epicrestally into the edentulous jaws of three minipigs and implant stability was assessed by measuring the implant stability quotient (ISQ). Prior to implantation half of the implants were photofunctionalized with intense UV-light. After three months, the implants were exposed and ISQ was measured again. After six months of implant exposure, the minipigs were sacrificed and the harvested specimens were analyzed using histomorphometric, light, and fluorescence microscopy.@*MAIN RESULTS@#Forty-two of 48 implants osseointegrated. The overall mean bone-implant contact area (BIC) was (64±22)%. No significant differences were found in BIC or ISQ value (multivariate analysis of variance (MANOVA), P>0.05) between implants with and without exposure to UV photofunctionalization.@*CONCLUSIONS@#No significant effects were observed on osseointegration of dental titanium implants nine months after exposure of UV photofunctionalization.


Subject(s)
Animals , Female , Male , Dental Implantation, Endosseous , Methods , Dental Implants , Equipment Failure Analysis , Hydrophobic and Hydrophilic Interactions , Models, Animal , Osseointegration , Surface Properties , Swine , Swine, Miniature , Titanium , Ultraviolet Rays
2.
Anatomy & Cell Biology ; : 85-94, 2015.
Article in English | WPRIM | ID: wpr-23348

ABSTRACT

To date there is no sufficient in vitro fat tissue engineering and a protocol has not been well established for this purpose. Therefore, we evaluated the in vitro influence of two different adipogenic growth media for their stimulation potential on different cell lineages to clearly define the most potent adipogenic growth media for future in vitro tissue engineering approaches. The samples for differentiation were composed of human adipogenic-derived stroma cells (hADSCs) and human bone marrow mesenchymal stroma cells (hMSCs). A normal adipogenic medium (NAM) and a specific adipogenic medium (SAM) were tested for their adipogenic stimulation potential. After 10 days and 21 days the relative gene expression was measured for the adipogenic marker genes PPARgamma2, C/EBPalpha, FABP4, LPL, and GLUT4 detected through real time reverse transcriptase polymease chain reaction (RT-PCR). Other study variables were the comparison between NAM and SAM and between the used cells hADSCs and hMSCs. Additionally an Oil-Red staining was performed after 21 days. Our results revealed that only SAM was significantly (P<0.05) superior in the differentiation process in contrast to NAM for 10 days and 21 days. As well was SAM superior to differentiate the used cell lineages. This was evaluated by the detected marker genes PPARgamma2, C/EBPalpha, FABP4, LPL, and GLUT4 through real time RT-PCR and by Oil-Red staining. In addition, the hMSCs proofed to be equal donor cells for adipogenic differentiation especially when stimulated by SAM. The results suggest that the SAM should be established as a new standard medium for a more promising in vitro adipogenic differentiation.


Subject(s)
Humans , Bone Marrow , Cell Culture Techniques , Cell Lineage , Gene Expression , PPAR gamma , RNA-Directed DNA Polymerase , Tissue Donors , Tissue Engineering
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