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1.
Indian J Med Sci ; 2008 Dec; 62(12): 492-5
Article in English | IMSEAR | ID: sea-67591

ABSTRACT

S. pneumoniae continues to be a major cause of invasive disease worldwide with considerable mortality and morbidity. Here we report the emergence of penicillin intermediate resistance to S. pneumoniae in India, which may predispose for an increased incidence of invasive pneumococcal disease in both children and adults with multi-drug resistance profile resulting in clinical failure.

2.
Southeast Asian J Trop Med Public Health ; 2004 Dec; 35(4): 828-33
Article in English | IMSEAR | ID: sea-34767

ABSTRACT

An indirect enzyme linked immunosorbent assay (ELISA) using monoclonal antibody (MAb) originated from the native Thai isolates of P. vivax (McPV1) and the polyclonal antibody (PAb) raised against Nepali isolates of P. vivax was developed for detection of P vivax antigens in red cell lysates. The assay was specific (100%) since it was positive only with P. vivax-infected erythrocytes and was negative when erythrocytes from 40 healthy individuals from malaria non-endemic areas and 40 P. falciparum infected erythrocytes were tested. When the assay was applied to 203 vivax blood samples already proven by microscopic examination collected from Dhanusha district of Nepal, and using the cut-off level of the mean optical density (OD) (0.144) of 40 healthy individuals who had been living in malaria-endemic areas (0.073) + 2 SD (0.016), the assay could detect 189/203 samples, indicating the sensitivity of the test was 93.1% with a detection limit of erythrocytes of 240 parasites/10(6) erythrocytes. In addition, the assay was negative when 40 blood samples with fever of unknown origin, collected from the same malaria-endemic areas, were tested. However, there was a significant correlation between OD values and parasitemia (r=0.649; p=0.018). The results indicate that MAb-PAb indirect ELISA using MAb raised against Thai isolates of P. vivax as the coating antibodies, and polyclonal antibodies raised against local Nepali isolates as the detecting antibody, could detect P. vivax antigens with high degrees of sensitivity and specificity. Furthermore, it seems that the McPV1 MAb raised against Thai isolates of P. vivax could recognize the antigens of Nepali isolates in a wide range of blood samples.


Subject(s)
Adolescent , Adult , Aged , Animals , Antibodies, Monoclonal/immunology , Antigens, Protozoan/blood , Case-Control Studies , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Humans , Infant , Middle Aged , Nepal , Plasmodium vivax/immunology , Thailand
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