ABSTRACT
Autosomal dominant polycystic kidney disease (ADPKD), one of the most common human monogenic diseases, is characterized by the presence of numerous fluid-filled renal cysts and is a leading cause of end-stage renal disease (ESRD). Urinary biomarkers may be useful for predicting the variable course of ADPKD progression from cyst growth to ESRD. Methods: To identify candidate urinary biomarkers of ADPKD progression, we used CRISPR/Cas9 genome editing to generate porcine fibroblasts with mono- and biallelic ADPKD gene knockout (PKD2+/– and PKD2–/–, respectively). We then performed RNA-sequencing analysis on these cells. Results: Levels of osteopontin (OPN), which is expressed by renal epithelial tubular cells and excreted into urine, were reduced in PKD2–/– cells but not in PKD2+/– cells. OPN levels were also reduced in the renal cyst cells of ADPKD patients. Next, we investigated whether OPN excretion was decreased in patients with ADPKD via enzyme-linked immunosorbent assay. OPN levels excreted into renal cyst cell culture media and urine from ADPKD patients were decreased. To investigate whether OPN can predict the rate of ADPKD progression, we compared urinary excretion of OPN in ADPKD patients with slow progression and those with rapid progression. Those with rapid progression had an estimated glomerular filtration rate of >60 mL/min/1.73 m2 . Urinary OPN excretion levels were lower in rapid progressors than in slow progressors. Conclusion: These findings suggest that OPN is a useful urinary biomarker for predicting ADPKD progression.
ABSTRACT
PURPOSE: The purposes of this study was to identify the effects of nursing practice environment, compassion fatigue and compassion satisfaction on burnout in clinical nurses. METHODS: Participants selected for the final analysis were 208 nurses working in 2 general hospitals in Busan and Masan. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson correlation and Hierarchial Multiple Regression with SPSS/WIN 18.0. RESULTS: Compassion fatigue had a significant positive effect on burnout, while Compassion satisfaction had a negative effect on burnout, but Nursing practice environment had no effect on burnout. The explained variance for burnout was 61% and compassion satisfaction was the most significant factor in burnout of nurses. CONCLUSION: The results of this study indicate that to reduce burnout in clinical nurses it is necessary to develop programs to increase nurses' compassion satisfaction and decrease compassion fatigue.