ABSTRACT
Objective To investigate the correlation between leptin receptor(LEPR)gene Gln223Arg variation with metabo-lism syndrome and its influence on ambulatory blood pressure .Methods Totally 167 patients with metabolism syndrome were se-lected and contemporaneous 216 individuals undergoing the physical examination were selected as the control group .The blood pres-sure ,ambulatory blood pressure ,biochemical indicators and insulin were detected in all the subjects .The DNA polymorphology a-nalysis was performed by adopteint PCR—restricted fragment length polymorphism(RFLP) .The Gln223Arg genotype was judged by electrophoresis and sequencing .Results Three genotypes of AA ,GG and AG were detected .The frequency of carrying A alleles in the metabolism syndrome group was significantly higher than that in the control group .The occurrence risk of metabolism syn-drome and non—dipper type blood pressure rhythm for carrying allele A was 3 .302 times(P=0 .000;95% CI:2 .432 —4 .483)and 2 .506 times of carrying allele G(P=0 .000 ;95% CI:1 .566 —4 .008) .The patients with AA genotype had higher BMI ,blood pres-sure ,blood glucose and fasting insulin levels ,more serious dyslipidemia ,greater waist circumference and higher insulin resistance in-dex .The patients with metabolism syndrome carrying A allele also had higher ambulatory blood pressure indexes .Conclusion LEPR gene Gln223Arg polymorphism A allele carrier has the great risk for metabolism syndrome occurrence ,higher ambulatory blood pressure ,moreover is more inclined to non—dipper type blood pressure rhythm .
ABSTRACT
Objective: To explore the relationship between 1eptin receptor gene Gln223Arg polymorphism and metabolism syndrome (MS) with its impact on cardiac structure and function. Methods: Our research included 2 groups:MS group, n=167 patients with ifrst diagnosed MS without treatment in our hospital from 2005-10 to 2008-6 and Control group, n=216 healthy subjects from regular physical examination. Blood pressure, biochemical features, insulin levels and echocardiography were detected;leptin receptor Gln223Arg genotypes were measured by PCR-RFLP;the above indexes were compared between 2 groups. Results:The patients in MS group had the higher frequency of A allele than Control group. The MS occurrence rate in allele A carrier was 3.302 times higher than allele G carrier (P=0.000;95%CI 2.432-4.483). The patients in MS group already had left ventricular hypertrophy and impaired diastolic function. Compared with MS G allele carriers, the A allele carriers had the higher BMI, blood pressure, glucose, fasting glucose and insulin levels, longer waist circumference, more serious dyslipidemia and insulin resistance, left ventricular hypertrophy and impaired diastolic function. Conclusion: Leptin receptor gene Gln223Arg polymorphism is associated with the increased risk of MS occurrence and left ventricular hypertrophy.
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Objective To investigate the therapeutic effects of alendronate combined with Caltrate D on regional bone mineral density (BMD) in elderly type 2 diabetic women with osteoporosis. Methods Thirty-four elderly type 2 diabetic women with osteoporosis aged 60-79 years (average 68. 0±4.5 years), with body mass index (BMI) of (25.0±3.7) kg/cm2 and duration of diabetes (8.9 ±4.4) years were enrolled. At the basis of a comprehensive management, the patients were treated with alendronate combined with Caltrate D for 6 months. BMD of the lumbar spine and hip, several biochemical indexes were measured before and after treatment. Results After the 6 months treatment, the BMD of different regions at lumbar spine and hip all increased. The T value and BMD were significantly higher at lumbar spines than at hip (both P＜0.01). The differences of T value and BMD of different regions were significant (P = 0.003 and 0.005,respectively). BMD percent change at lumbar spine were L4 (44.7%) ＞ L3 (31.9%) ＞ L total (27.3 % ) ＞ L1 (20.0%) ＞ L2 (14.3 % ), and the BMD percent changes were significantly higher in L3 and L4 than in other regions at lumbar spine (P=0. 038 and 0. 008, respectively). The changes of T value and BMD were significantly higher in L3, L total, L1 and L4 than in L2 (T value: P=0. 036,0. 042, 0. 006 and 0. 004, respectively; BMD: P=0. 002, 0. 002, 0. 003 and 0. 001, respectively).Conclusions On the basis of comprehensive management, the 6 months treatment with alendronate combined with Caltrate D in elderly type 2 diabetic women with osteoporosis may achieve good therapeutic effect at lumbar spines, especially at the lower lumbar spines, while less effective at hip.
ABSTRACT
Objective To investigate the association of leptin receptor gene Gln223Arg polymorphism with metabolism syndrome and insulin resistance. Methods The genotypes of leptin receptor in 167 patients with metabolic syndrome and 216 healthy subjects were determined by polymerase chain reaction restriction fragment length polymorphism. The biochemical indicators were detected. Results The frequency of A alleles was significantly higher in patients with metabolic syndrome than in healthy subjects. The risk of metabolic syndrome in inviduals with allele A was 3. 302 folds of that with allele G( P<0.01 ). Compared with allele G, the patients withA allele had higher body mass index,blood pressure, blood glucose, insulin level, waist circumference, and more serious dyslipidemia. The risk of insulin resistance in patients with A allele was 3. 446 folds of that with allele G (P<0.01). Conclusions Leptin receptor gene Gln223Arg polymorphism is associated with an increased risk of metabolic syndrome and insulin resistance.