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Self-designed experiments were used to study the effects on developing the students'ability and improving teaching quality.The results show that self-designed experiments can improve students'learning interest, motivation,coordination,and research capabilities.
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This paper aims to raise concerns about the modification of bovine hemoglobin with the use of SC-mPEG as modifying reagent. The yield rates of SC-mPEG-BHB under dissimilar conditions are studied, and more important, a series of methods used to characterize the SC-mPEG-BHB mixtures are built. The best reactive system has been confirmed, i.e., at 4 degrees C, pH9; SC-mPEG and BHB are mixed according to the proportion of 60:1 and be submitted to reaction for 2 h.
Subject(s)
Animals , Cattle , Humans , Blood Substitutes , Hemoglobins , Chemistry , Classification , Pharmacology , Hydrogen-Ion Concentration , Polyethylene Glycols , Chemistry , ClassificationABSTRACT
OBJECTIVE: To study the reaction yield of SC-mPEG with bovine hemoglobin(BHB) under different conditions so as to establish a method for the analysis of the SC-mPEG-BHB mixtures.METHODS: The product was analyzed with the methods of SDS-PAGE,GPC,CE,TNBS,etc.The optimal reaction system for SC-mPEG with BHB was established based on the effects of different factors on SC-mPEG-modified BHB.RESULTS: The optimum reaction conditions for SC-mPEG with BHB were established as follows: SC-mPEG and BHB were mixed at an optimal ratio of 60∶1 and the reaction was lasted for 2 h in(pH9 and 4 ℃) buffer solution.CONCLUSIONS: This study established a foundation for the following study of red blood cell substitutes.
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Objectives to evaluate the quality of examination paper of natural product chemistry of clinical pharmacy for Grade 2005 so as to feed back teaching information and provide gist for improving the teaching quality.Methods Selecting examination paper of all the students of clinical pharmacy to analyze.Results The examination achievement accords with normal distribution with average(78.64?11.12),difficulty 0.62 and discrimination 0.40.Conclusion The model of the paper has the full-scale distribution,the discrimination is good,and it evaluates the mastery level of the students quite objectively.The training of students analysis ability and comprehensive judging ability should be strengthened in the future.
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OBJECTIVE: Researching the appropriate methods to separation,purification and detection the mixture of PEG modifier SC-mPEG with BHB.METHODS: Adopting cation-exchange chromatography and organic solvent precipitation to separate the mixture,then detecting it by SDS-PAGE and TNBS methods.RESULTS: Modifer and non-modifer product were separation by cation-exchange chromatography.The average modification rates of two peak product from cation-exchange chromatography are 52.95% and 14.19% detected by TNBS methods.CONCLUSIONS: Cation-exchange chromatography methods could be to separate and purify the SC-mPEG-BHB,TNBS methods coule be to detect the average modification rate of the SC-mPEG-BHB.