ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between the expression of aquaporin 4 (AQP4) and slow transit constipation(STC).</p><p><b>METHODS</b>The expression of AQP4 in the ascending colon mucosa of 45 patients with STC was detected by immunohistochemistry. The transit time of stool was measured with barium sulfate suspensions. Stool was classified using Bristol stool chart. The difference between the pre- and post-operation groups was analyzed.</p><p><b>RESULTS</b>Of 45 STC patients, 36 had high AQP4 expression (high expression group) and 9 had low AQP4 expression(low expression group). Preoperatively 30(83.3%) patients in the high expression group and 3(3/9) patients in the low expression group presented dry and hard stool (type I( or II()(P<0.01). Postoperatively, stool pattern was improved in all the patients of high expression group. One patient in low expression group still presented dry and hard stool(P<0.01). Preoperatively the transit time was(127.3+/-28.2) h in high expression group and (64.2+/-12.9) h in low expression group(P<0.01). Postoperatively, the transit time was (17.3+/-7.0) h in high expression group and (28.0+/-12.6) h in low expression groups(P<0.01).</p><p><b>CONCLUSION</b>High expression of AQP4 accelerates the water absorption in colon mucosa and may be one of the crucial events in the development of STC.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Aquaporin 4 , Metabolism , Colon , Metabolism , Constipation , Metabolism , Gastrointestinal Transit , Intestinal Mucosa , MetabolismABSTRACT
<p><b>BACKGROUND</b>In patients suffering from acute pancreatitis, the pathogenesis is not completely understood, and several recent studies in vitro suggested that heat shock proteins might play an important role in cell signaling. To investigate the possible role of extracellular heat shock protein 70 (Hsp70) in pancreatitis, toll-like receptor-4 (TLR4)-deficient and wild-type mice were administered with exogenous Hsp70 during the course of cerulein-induced pancreatitis (CIP).</p><p><b>METHODS</b>Acute pancreatitis was induced by 5 intraperitoneal injections of cerulein at hourly intervals, and then treated with recombinant Hsp70 through the caudal vein 4 hours after the start of cerulein injections. Subsequently serum amylase and serum cytokines levels were detected. Histologic alteration of the pancreas was evaluated. Tumor necrosis factor alpha (TNF-alpha) concentrations and myeloperoxidase (MPO) activity in both pancreas and lungs were analyzed. The nuclear factor kappa B (NF-kappaB) activation in pancreatic tissue was measured using a sensitive RelA enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Treatment with recombinant Hsp70 to wild-type mice in CIP resulted in significant aggravation of inflammation in pancreas, elevated levels of serum cytokines, up-regulation of pulmonary MPO activity and increase of lung tissues TNF-alpha concentrations. In contrast, treatment with Hsp70 to TLR4-deficient mice had little effect on serum cytokines levels, pancreatic inflammation, pulmonary MPO activity and TNF-alpha concentrations.</p><p><b>CONCLUSIONS</b>The results suggest that extracellular Hsp70 might induce systemic inflammatory response syndrome (SIRS)-like response in vivo and TLR4 might be involved in the Hsp70-mediated activation of inflammatory reaction in the progression of CIP without infection.</p>
Subject(s)
Animals , Female , Male , Mice , Acute Disease , Ceruletide , Toxicity , HSP70 Heat-Shock Proteins , Physiology , Mice, Inbred C57BL , Pancreatitis , Systemic Inflammatory Response Syndrome , Toll-Like Receptor 4 , PhysiologyABSTRACT
Objective To detect EIA factor expression in human rectal cancer and normal tissue and to determine whether it is correlated with invasion and metastasis of human rectal cancer. Methods Real- time RT-PCR was used to detect E1AF expression in matched rectal cancers and normal tissues from g6 in- patients.Results Among the 86 rectal cancer samples tested,55 cases E1AF mRNA overexpression was ob- served. The mRNA expression of E1AF in the sample group was remarkably different from that in the control group.In carcinomas,E1AF mRNA expression correlated significantly with histological type,depth of inva- sion,lymph node and distant metastasis and advanced Duke stage.Conclusion E1AF is correlated signifi- cantly with invasion and metastasis of human rectal cancer and may be an important factor in the invasion and metastasis.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between cyclin D2 and P210(BCR/ABL) tyrosine kinase in chronic myelogenous leukemia (CML).</p><p><b>METHODS</b>RT-PCR, Western blot and flow cytometry were performed to detect the expression of cyclin D2 in K562 cells and in K562-ib-eGFP cells which express intracellular single-chain antibody (sFv, intrabody) against ABL tyrosine kinase domain.</p><p><b>RESULTS</b>Cyclin D2 expression in K562-ib-eGFP cells was 18.90% which was lower than that of control K562 cells (48.10%), and the number of S-phase cells in K562-ib-eGFP was 40.40% which was much lower than that in K562 cells (64.34%).</p><p><b>CONCLUSION</b>Cyclin D2 is a potential down-stream signal molecule of the p210(BCR/ABL) tyrosine kinase in CML. The altered expression of cyclin D2 may contribute to the over proliferation of CML cells.</p>
Subject(s)
Humans , Blotting, Western , Cyclin D2 , Cyclins , Genetics , Flow Cytometry , Genes, abl , K562 Cells , Leukemia, Myelogenous, Chronic, BCR-ABL Positive , Metabolism , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
<p><b>OBJECTIVE</b>To study the effects of anti-ABL tyrosine kinase intrabody on the growth of human chronic myelogenous leukemia (CML) cells in nude mice.</p><p><b>METHODS</b>A recombinant retroviral vector MSCV-ibE-IRES-eGFP was constructed to express intracellular single-chain antibody (intrabody) against ABL tyrosine kinase domain in CML cells. K562 cells were transduced with the retrovirus, eGFP+ cells were then selected by fluorescence-activated cell sorting (FACS). The intrabody mRNA expression was determined by reverse transcription (RT)-polymerase chain reaction (PCR). BCR/ABL and c-ABL protein tyrosine kinase (PTK) activity in the cells was examined. Transduced cells and control group K562 cells were transplanted into nude mice respectively and the tumor sizes were dynamically observed.</p><p><b>RESULTS</b>K562-ibE cell was obtained. Expression of the BCR/ABL and c-ABL protein tyrosine kinase activity of harvested K562-ibE cells were markedly inhibited. At 14, 21 and 28 days after cell injection, the tumor volumes of experimental mice were obviously smaller than that of control mice, about one half of the control groups (P < 0.05).</p><p><b>CONCLUSION</b>The growth of K562-ibE cells was significantly inhibited in vivo. It is possible that inhibition of the BCR/ABL protein tyrosine kinase activity by the intrabody blocked BCR/ABL signal transduction pathway, promoted apoptosis and reduced tumorigenicity of K562 cells in vivo.</p>
Subject(s)
Animals , Humans , Mice , Antibodies , Genetics , Apoptosis , Cell Division , Fusion Proteins, bcr-abl , Genetics , Allergy and Immunology , Genetic Vectors , K562 Cells , Mice, Nude , Neoplasm Transplantation , Protein-Tyrosine Kinases , Allergy and Immunology , Metabolism , Proto-Oncogene Proteins c-abl , Genetics , Allergy and Immunology , Retroviridae , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To develop an in vitro assay that allows the culture and identification of a single human bone marrow progenitor closely related to hematopoietic stem cell, which is more primitive than LTC-IC, and to find an efficient culture conditions for NK-IC expansion.</p><p><b>METHODS</b>Fusion protein IL6/IL2 was reconstructed and expressed in E. coli DH5 alpha. ML-IC was determined by watching if the single cell can give rise to secondary progenitors with both LTC-IC and NK-IC characteristics. LTC-IC frequency was determined by the CFC clonogenic methylcellulose assay. NK-IC frequency was determined by phenotyping CD56 positive NK cells. The effect of FPIL6/IL2 on the expansion of NK-IC was examined by comparing the colony number of NK cells before and after the culture.</p><p><b>RESULTS</b>After the initial 4-week expansion culture, we showed that (25.75 +/- 5.68)% of freshly sorted Lin-/34+/DRdim cells were able to generate functional NK-IC in one or more secondary FPIL6/IL2 cultures, whereas (6.81 +/- 1.97)% in the control. A total of 102 NK-IC cells were present when were cultured for 6-7 weeks in FPIL6/IL2 expansion medium, which was much higher than the 33 NK-IC cells in the control.</p><p><b>CONCLUSION</b>ML-IC assay will prove useful to assess a very primitive hematopoietic cell with multilineage generative capacity. FPIL6/IL2 is capable of initiating and promoting NK-IC expansion greatly in ex vivo cultures in terms of net-conservation and net proliferation.</p>
Subject(s)
Animals , Humans , Mice , Biological Assay , Cell Culture Techniques , Methods , Cell Differentiation , Cell Division , Cells, Cultured , Coculture Techniques , Methods , Hematopoietic Stem Cells , Cell Biology , Interleukin-2 , Genetics , Pharmacology , Interleukin-6 , Genetics , Pharmacology , Killer Cells, Natural , Cell Biology , Metabolism , Recombinant Fusion Proteins , Pharmacology , Stromal Cells , Cell BiologyABSTRACT
Avulsion injury of the flexor digitorum profundus tendon from distal phalanx is considered as a rare injury. Accrording to the classification by Leddy and Pacter, this case is Type III, which is a large bony fragment retained by the tendon. The distal pulley prevents retraction beyond the middle phalanx. We are reporting a case with brief review of literatures.