Interleukin?22 promotes proliferation of fibroblast?like synoviocytes from patients with rheumatoid arthritis by inducing STAT3 phosphorylation / 南方医科大学学报

<p><b>OBJECTIVE</b>To clarify the mechanism by which interleukin?22 (IL?22) promotes the proliferation of fibroblast?like synoviocytes (FLS) from patients with rheumatoid arthritis (RA).</p><p><b>METHODS</b>FLS were isolated from the synovial tissues of patients with RA and identified by immunohistochemistry for vimentin/CD68. The cells were subcultured and incubated with different concentrations of IL?22 for 24, 48, or 72 h, and their proliferation was examined using MTT assay. After treatment of the cells with IL?22 and AG490, alone or in combination, the expressions of the total and phosphorylated proteins of STAT3, ERK1/2 and P38 were detected with Western blotting.</p><p><b>RESULTS</b>IL?22 significantly increased the proliferation of FLS in a dose?dependent manner (P<0.05). The total protein of STAT3 in the cells showed no significant changes with extended time of IL?22 treatment (P=0.68), but the expression of phosphorylated STAT3 protein increased significantly (P<0.001). The total and phosphorylated proteins of ERK1/2 and P38 underwent no significant changes after IL?22 treatment (P>0.05). A combined treatment with 50 ng/mL IL?22 and 100 µmol/L AG490 resulted in a significant decrease in the proliferation of FLS as compared with IL?22 treatment alone (P<0.01).</p><p><b>CONCLUSION</b>IL?22 can dose?dependently promote the proliferation of FLS from patients with RA by inducing phosphorylation of STAT3 protein but not through ERK1/2 or P38 signal pathway.</p>

Analysis on Mortality Rate and Cause of Injury among the Urban and Rural Residents in Hebei Province during 2004—2005 / 中国慢性病预防与控制

Objective To understand the mortality rate and cause of the injury in Hebei province in order to provide scientific theoretical basis for drawing up effective prevention measures.Methods The injury deaths data was collected from 18 surveillance spots in Hebei province during 2004—2005,and the injury was classified with ICD-10 and the corresponding mortality rates were calculated.Results The average injury mortality rate was 54.02/100 000(the age-adjusted rate was 51.62/100 000).It was the 5 th-ranked cause of death.The leading five causes of injury were traffic injury by motor vehicles, traffic injury by non-motor vehicles,suicide,poisoning and fall,which accounted for 23.96%,22.05%,13.10%,11.99%, 5.62%,respectively.The average injury mortality rate in rural(58.48/100 000)was higher than in urban(31.60/100 000)(?~2= 255.82,P