ABSTRACT
OBJECTIVE@#The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.@*METHOD@#Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.@*RESULT@#We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.@*CONCLUSION@#TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
Subject(s)
Humans , DNA Primers , DNA, Plant/isolation & purification , Feasibility Studies , Papaver/genetics , Plant Leaves/genetics , Polymerase Chain Reaction , Random Amplified Polymorphic DNA Technique/methods , Species SpecificityABSTRACT
Somatic gene therapy based on nonviral and viral vectors is an attractive approach for treatment of human diseases. It has developed from preliminary animal experiments to clinical trials. Vectors and target genes used in gene therapy are mainly focused on viral, nonviral vector and single target gene or monogene and each vector system has a series of advantages and limitations. However, the application of gene therapy to human disease is currently hampered by potential hazards of methods of gene delivery, the relatively low efficiency and intracellular stability of target gene. Safety, efficacy and stability are three problems that people often met and also three principles that people should be followed in gene therapy.