Expression and clinical significance of syndecan-1 mRNA and HPA-1 mRNA in colorectal cancer detected with real-time fluorescent quantitative polymerase chain reaction / 癌症

<p><b>BACKGROUND AND OBJECTIVE</b>Invasion and metastasis are the most common causes of mortality for patients with colorectal neoplasms, and blocking invasion and metastasis in a timely fashion has become a hot research focus. We investigated the expression of the messenger RNA of Syndecan-1 and HPA-1 in colorectal cancer, and their correlation with invasion and metastasis.</p><p><b>METHODS</b>Real-time fluorescent quantitative polymerase chain reaction (PCR) was used to detect the expression of Syndecan-1 and HPA-1 in specimens from 49 patients with colorectal cancer, 49 paired adjacent colorectal neoplasms (2 cm from the carcinoma), and 49 surgical margins of paired normal colorectal mucosa tissue (5 cm from the carcinoma), to analyze their correlation with clinicopathologic characteristics of colorectal neoplasm.</p><p><b>RESULTS</b>The expression of HPA-1 mRNA was significantly higher in colorectal cancer (40.56 +/- 11.75) than that in the paired adjacent colorectal neoplasms (18.28 +/- 11.33) and normal colorectal mucosa tissue (10.80 +/- 10.20) (all P < 0.001). The expression of HPA-1 mRNA was significantly higher in paired adjacent colorectal neoplasms than that in normal colorectal mucosa (P < 0.05). The expression of Syndecan-1 mRNA was significantly higher in normal colorectal mucosa (61.21 +/- 12.96) than in the paired adjacent mucosa (14.35 +/- 11.06) or colorectal cancer (10.12 +/- 8.58) (all P < 0.001). The expression of Syndecan-1 mRNA was significantly higher in the paired adjacent mucosa than that in colorectal cancer (P < 0.05). The decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 were closely associated with the degree of differentiation, the depth of infiltration, lymph node metastasis, vessel metastasis, and TNM staging of colorectal cancer (all P < 0.05). Spearman rank correlation analysis demonstrated a significant correlation between Syndecan-1 and HPA-1(r = -0.405, P < 0.05).</p><p><b>CONCLUSIONS</b>The expression of Syndecan-1 mRNA was significantly highest in normal colorectal mucosa and the expression of HPA-1 mRNA was significantly highest in colorectal cancer. At the same time, the decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 mRNA can promote the invasion and metastasis of colorectal cancer. The determination of Syndecan-1 and HPA-1 may be of value in the treatment as well as in the prognosis of patients with colorectal cancer.</p>

Adjuvant effect of transcutaneous electrical acupoint stimulation in propofol-fentanyl anesthesia in partial mastectomy / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the adjuvant effect of transcutaneous electrical acupoint stimulation (TEAS) in propofol-fentanyl anesthesia in partial mastectomy.</p><p><b>METHODS</b>Ninety patients undergoing partial mastectomy were randomly divided into 3 groups (n=30), namely group A with propofol-fentanyl anesthesia (PFVA), group B with PFVA and TEAS at Hegu-Laogong and Neiguan-WaiGuan acupoints, and group C with PFVA and TEAS at Hegu-Laogong, Neiguan-Waiguan, and bilateral Jianjing acupoints. The bispectral index (BIS), heart rate (HR), saturation of pulse oxygen (SpO2), blood pressure (BP), the reaction of patients to the incision and awakening quality were observed at different time points. Blood samples were obtained from the dorsal artery of the foot to determine the levels of adrenaline and beta-endorphin using the enzyme-linked immunosorbent assay before TEAS (entry into the OR in group A) and at 30 min after TEAS (30 min after entry into the OR in group A), 5 min after incision, end of the surgery and awakening.</p><p><b>RESULTS</b>Thirty minutes after TEAS (or 30 min after entry into the OR in group A), BIS, BP and HR were all decreased significantly in groups B and C (P<0.05) but remained stable in group A (P>0.05). The plasma propofol concentration of groups B and C were significantly lower than that in group A, and group B had the highest plasma propofol concentration (P<0.05). The total dose of propofol in groups B and C were decreased by 19% and 27% in comparison with that in group A, respectively. Compared with the basal value, the content of beta-endorphin in groups B and C increased obviously 30 min after TEAS, being the highest in group C (P<0.05); the adrenaline level in groups B and C remained stable after TEAS (P>0.05).</p><p><b>CONCLUSION</b>TEAS provides an adjuvant effect in propofol-fentanyl anesthesia and obviously enhances the analgesia effect.</p>