ABSTRACT
Objective:To explore role of miR-146a in regulating TLR4/NF-κB pathway on inflammatory injury and neuropro-tection in intracerebral hemorrhage model rats and its possible mechanism.Methods:A total of 40 rats were selected and randomly divided into sham,model,over-expressing miR-146a adenovirus and negative virus injection groups,with 10 rats in each group.Garcia score was used for neurological function;HE staining was used to observe changes of brain tissues.ELISA was used to detect inflammatory factors levels.TLR4,NF-κB protein and gene expressions in brain tissues were detected by Western blot and RT-PCR.Results:Compared with model group,neural function score of overexpressed miR-146a adenovirus injection group was increased(P<0.05).Model group had abnormal cell morphology,edema and inflammation.Cell morphology,edema and inflammation were alleviated in overexpressed miR-146a adenovirus injection group.Inflammatory factors levels in model group were higher than sham group(P<0.05).Inflammatory factors levels in overexpressed miR-146a adenovirus injection group were lower than model group(P<0.05).TLR4,NF-κB protein and mRNA expressions in model group were increased than sham group(P<0.05).TLR4,NF-κB protein and mRNA expressions in overexpressed miR-146a adenovirus injection group were decreased than model group(P<0.05).Conclusion:miR-146a can improve neural function and reduce inflammatory injury in rats with intracerebral hemorrhage,possibly by inhibiting activation of TLR4/NF-κB signaling pathway and reducing inflammatory factors levels of brain tissues.
ABSTRACT
Objective To compare the clinical effects of three different methods (temporalis restoration or not) used in standard kull defect repairs after standard decompressive craniectomy.Methods Seventy-one patients with kull defects were chosen in our study; 31 of them,admitted to our hospital from January 2012 to December 2013,adopted repairs by temporalis restoration+digital titanium mesh shaping technology (group A); 21 of them,admitted to our hospital from January 2010 to December 2011,adopted repairs under temporalis+digital titanium mesh shaping technology (group B); and 19 of them,admitted to our hospital from June 2008 to December 2009,adopted repairs outside temporalis+digital titanium mesh shaping technology (group C).The operation time,blood loss,postoperative complications and subjective symptoms such as poor comfort adverse reactions in the three groups were compared.Results The mean operation time:group A was (80±10.15) min,group B (76± 9.25) min and group C (72±11.5) min,without significant differences (P＞0.05); average amount of bleeding:group A was (75±9) mL,group B (70±11) mL and group C (68±8) mL,without significant differences (P＞0.05).Adverse reactions were noted in 6 patients of group A (13 [68.42％] complained uncomfortable conditions),7 of group B (7 [33.3％] complained uncomfortable conditions) and 3 of group C (4 [12.9％] complained uncomfortable conditions),with statistically significant differences (P＜0.05).Conclusion Skull repairs bv temporalis restoration+digital titanium mesh shaping technology,being more similar to the physical structure of the patients,enjoy higher postoperative patient satisfaction,more satisfactory functional recovery,and lower rate of adverse reactions,and had better effect than the other two methods.