miR-155-5p Promotes Invasion and Migration of MCF-7 Breast Cancer Cell by Down-regulating SOX1 / 华中科技大学学报(医学版)

Objective To examine the mechanism by which miR-155-5p regulates sex determining region Y-boc 1(SOX1) expression in breast cancer cell line MCF-7,and to investigate the effect of miR-155-5p on the invasion and metastasis of these cells.Methods After up-regulation or down-regulation of the expression of miR-155-5p in MCF-7 cells,the transwell assay was performed to detect the invasive and migrating ability of MCF-7 cells;SOX1 mRNA expression and protein levels were detected by real-time PCR and Western blot,respectively;immunofluorescence was used to test the distribution and levels of SOX1 pro-tein,and dual luciferase reporter experiments to discover the binding of miR-155-5p to the 3′-UTR region of SOX1 mR-NA.Results Up-regulating the expression of miR-155-5p could enhance the migrating and invasive ability of MCF-7 cells and simultaneously decrease the mRNA and protein levels of SOX1(P <0.05).On the contrary,inhibition of miR-155-5p depressed the invasion and migration of MCF-7 cells and increased the expression of SOX1(P < 0.05).Dual luciferase reporter experi-ments confirmed that miR-155-5p could bind to the specific sequence in the 3′-UTR of SOX1 mRNA and play a role of regula-tor.Conclusion miR-155-5p promotes the invasion and migration of breast cancer cell line MCF-7 via inhibiting the expression of SOX1.

Transanal laparoscopic radical resection with telescopic anastomosis for low rectal cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To assess the safety, feasibility and clinical outcome of laparoscopic radical resection for low rectal cancer with telescopic anastomosis or with colostomy by stapler through transanal resection without abdominal incisions.</p><p><b>METHODS</b>From January 2010 to September 2014, 37 patients underwent laparoscopic radical resection for low rectal cancer through transanal resection without abdominal incisions. The tumors were 4-7 cm above the anal verge. On preoperative assessment, 26 cases were T1N0M0 and 11 were T2N0M0.</p><p><b>RESULTS</b>For all cases, successful surgery was performed. In telescopic anastomosis group, the mean operative time was (178±21) min, with average blood loss of (76±11) ml and (13±7) lymph nodes harvested. Return of bowel function was (3.0±1.2) d and the hospital stay was (12.0±4.2) d without postoperative complications. Patients were followed up for 3-45 months. Twelve months after surgery, 94.6%(35/37) patients achieved anal function Kirwan grade 1, indicating that their anal function returned to normal.</p><p><b>CONCLUSIONS</b>Laparoscopic radical resection for low rectal cancer with telescopic anastomosis or colostomy by stapler through transanal resection without abdominal incisions is safe and feasible. Satisfactory clinical outcome can be achieved mini-invasively.</p>

Chemoradiation effect of combined preoperative intensity-modulated radiotherapy with oral capecitabine in patients with locally advanced mid-low rectal cancer using a simultaneous integrated boost of tomotherapy / 中国综合临床

Objective To assess the safety and efficacy of preoperative intensity-modulated radiotherapy(IMRT) with oral capecitabine in patients with locally advanced mid-low rectal cancer using a simultaneous integrated boost (SIB) of tomotherapy.Methods Total 16 patients with resectable locally advanced mid-low rectal cancer (patients with T3 to T4 and/or N ± rectal cancer) were enroll in current study.Patients were received IMRT to 2 dose levels simultaneously (55 and 47.5 Gy in 25 fractions) with concurrent capecitabine 825 mg/m2 twice daily,5 days/week.Total mesorectal excision was performed at 8 to 9 week after the completion of chemoradiation.The primary end point included side effect,the rate of sphinctersparing,postoperative complication and pathological complete response rate (pCR) were observed.Side effects were scored using the National Cancer Institute Common Terminology Criteria for Adverse Events version 3.0.Results All patients were received chemoradiotion therapy without any break.Tomotherapy showed superiority with respect to target coverage,homogeneity and conformality.Two patients refused to perform radical surgery because of almost complete primary tumor regression and complete symptom relief after neoadjuvant therapy.Fourteen patients underwent surgical resection and 11 patients (78.6％) underwent sphincter-sparing lower anterior resection.Four patients(28.6％) had a pathological complete response.The incidence of grade 1-2 hematologic,gastro-intestinal toxicities were 62.5％ (10/16) and 18.8％ (3/16).The incidence of grade 3 skin toxicities were 68.8％(10/16).Grade Ⅳ side effect was not observed.Surgical complications (incisional infection on thirteen after surgery) were observed in 1 patient.Conclusion Preoperative simultaneous integrated boost of tomotherapy with concurrent oral capecitabine is safe and well tolerated in patients with a promising local control.However,a larger number of patients and a long follow-up are required to assess its potential superiority.

Anus-preserving rectectomy using telescopic anastomosis for middle and lower rectal cancer, report of 402 cases / 中华普通外科杂志

Objective To study the clinical efficacy of anus-preserving rectectomy by using telescopic anastomosis of colon and rectal mucosa for the middle and lower rectal cancer. Methods A retrospective analysis was carried out in 402 cases with middle and lower rectal cancer undergoing telescopic anastomosis for anus-preserving procedure, including 241 males and 161 females, age ranging from 21 to 99 years, averaging at 55. 7 years. The distal margins of the tumors were within 6 - 9 cm to anal verge. According to TNM staging, there were 123 cases in Stage Ⅰ , 244 cases in Stage Ⅱ , 31 cases in Stage Ⅲ,and 4 cases in Stage Ⅳ. Results 345(345/402, 85. 8% ) cases were followed up, the median time of the follow-up was 6. 1 years. Postoperative complications included 17(4.2%) cases of stomal leakage, 11(2.7% ) cases of stomal stenosis. All patients recovered normal defecating function 12-24 weeks post operation. Local recurrence rate was 6. 3% (22/345). Hepatic and lung metastasis was 13. 6% (47/345) and 2. 6% (9/345)respectively. The five year survival rate was 68. 7% (112/163). Conclusions Anuspreserving rectectomy by using telescopic anastomosis is safe and effective procedure to treat middle and lower rectal cancer, with the preservation of anal function and without the increasing rate of local recurrence.