ABSTRACT
A fluorescence quenching method was established for the determination of H2 S in intestinal perfusate by optical fiber sensing technology combined with micro sequential injection lab_on_valve (μSIA_lov). In the experiment, 100 μL of 0. 1 mol/L NaOH was used as the carrier, and 50 μL of 5. 0×10-5 mol/L fluorescein mercury and 50 μL of sample were selected for the determination. The detection flow rate at the flowcell was 25 μL/s. According to H2 S quenching fluorescence at 521 nm, the concentration of H2 S in the sample was determined. The detected concentration range of H2S was 5. 0×10-6-8. 0×10-5 mol/L, and the detection limit was 5. 4×10-7 mol/L. Detection result of H2S in intestinal perfusion was 3. 8×10-5 mol/L with 3. 1% RSD (n=3). This method can be used for the determination of H2S effectively in the samples, which lay the foundation for real_time online measurement of H2 S in biological samples.
ABSTRACT
[Objective] To investigate the effects of Yangyin Kangdu Powder (YKP) on peripheral blood T-lymphocyte subsets levels in X-ray-irradiated mice. [Methods] Forty-five mice were randomized into normal control, model and YKP groups. Except the normal group, the rats in model and YKP groups were irradiated with 6Gy X-ray to establish models with acute radiation-induced injury. Normal saline was given to the model group while YKP in the dosage of 10 g?kg-1?d-1 was orally administered to YKP group for one week. The changes of CD4 and CD8 T-lymphocyte subsets levels were detected by flow cytometry. [Results] Plasma levels of CD4 and CD8 and the ratio of CD4/CD8 decreased in the model group, the difference being significant as compared with the normal control group (P
ABSTRACT
Objective:To explore the mechanism of traditional Chinese medicine compound (Yang Yin Kang Du powder) in radioprotection.To study the relationship between T lymhocytes and antioxidation of mice radiated with X ray.Methods:T lymphocyte subset of in peripheral blood mice was determined by indirect immunofluorescence and flow cytometer.To T AOC,Cat,SOD and MDA were anoly zedby chemical colorimetry.Results:Compared with the normal group,the percentage of CD4 and CD8 in two irradiated groups declined significantly( P