ABSTRACT
Objectives:In this study,the effects of glutamine(Gln) and branched chain amino acids(BCAA) enriched formulas on free radical metabolism and immunity in traumatized rats were investigated. Methods:After injury,twenty one male Wistar rats were randomly divided into three groups,and fed with rations containing casein,a commercial amino acids(17AA),and a new amino acids formula(20 AA) respectively.The rations were isonitrogenous and isocaloric.Before operation and on days 3,7,14 postoperation,body weight,dietary intake,the concentrations of MDA,the activities of SOD in plasma were measured.At last,the animals were killed,the hydroyproline and spleen lymphocyte blastogenesis were determined. Results:①After injury,body weight of rats were reduced significantly,the concentrations of MDA in plasma were increased,while the SOD activities were decreased. ②Compared with 17AA group, the levels of hydroyproline in sponge were increased in 20AA group.③There were better effects of reducing plasma MDA levels and enhancing plasma antioxidase activities in 20 AA group than 17 AA group.④The weight of thymus and spleen and spleen lymphocyte blastogenesis were more obviously increased in 20AA group than in 17AA group. Conclusions:The new amino acids preparations can increase the antioxidase activities,enhance immunity and promote wound healing.
ABSTRACT
Objective: To investigate the effect of quercetin on hepatic ischemia reperfusion injury (HIRI) in rats.Methods: Quercetin was administered intragastrically. Vitamin C was used as positive control. HIRI was induced by blocking and then releasing portal vein and hepatic artery in rats. The hepatic content of quercetin was analyzed by HPLC. Plasma GPT, GOT activities, MDA concentration and hepa-tic GSH and MDA contents, GSH-Px, SOD, XO activities, total antioxidant capacity (TAOC), ROS, DNA fragmentation were measured.Results: After HIRI, plasma GPT, GOT activities and MDA concentration were increased significantly. Hepatic GSH and TAOC were decreased remarkably. The activities of SOD and GSH-Px were significantly decreased while XO activity, ROS production and DNA fragmentation increased. Intragastric administration of quercetin increased hepatic quercetin and decreased ROS production and plasma activities of GPT, GOT and MDA concentration. Hepatic GSH and SOD, GSH-Px activities and TAOC also recovered remarkably, but no significant change in DNA fragmentation. Vitamin C was also effective in protecting against HIRI. Conclusion: Quercetin is effective against HIRI. The mechanism is associated with increased hepatic antioxidant capacity.