Periodontal and endodontic pathology delays extraction socket healing in a canine model

PURPOSE: The aim of the present exploratory study was to evaluate extraction socket healing at sites with a history of periodontal and endodontic pathology. METHODS: The mandibular 4th premolar teeth in 5 adult beagle dogs served as experimental units. Periodontal and endodontic lesions were induced in 1 premolar site in each animal using wire ligatures and pulpal exposure over 3 months (diseased sites). The contralateral premolar sites served as healthy controls. The mandibular 4th premolar teeth were then extracted with minimal trauma, followed by careful wound debridement. The animals were sacrificed at days 1, 7, 30, 60, and 90 post-extraction for analysis, and the healing patterns at the healthy and diseased extraction sites were compared using radiography, scanning electron microscopy, histology, and histometry. RESULTS: During the first 7 days of healing, a significant presence of inflammatory granulation tissue was noted at the diseased sites (day 1), along with a slightly accelerated rate of fibrin clot resolution on day 7. On day 30, the diseased extraction sites showed a greater percentage of persistent fibrous connective tissue, and an absence of bone marrow formation. In contrast, healthy sites showed initial signs of bone marrow formation on day 30, and subsequently a significantly greater proportion of mature bone marrow formation on both days 60 and 90. Radiographs exhibited sclerotic changes adjoining apical endodontic lesions, with scanning electron microscopy showing collapsed Volkmann canals protruding from these regions in the diseased sites. Furthermore, periodontal ligament fibers exhibited a parallel orientation to the alveolar walls of the diseased sites, in contrast to a perpendicular arrangement in the healthy sites. CONCLUSIONS: Within the limitations of this study, it appears that a history of periodontal and endodontic pathology may critically affect bone formation and maturation, leading to delayed and compromised extraction socket healing.

Preclinical development of a humanized neutralizing antibody targeting HGF

Hepatocyte growth factor (HGF) and its receptor, cMET, play critical roles in cell proliferation, angiogenesis and invasion in a wide variety of cancers. We therefore examined the anti-tumor activity of the humanized monoclonal anti-HGF antibody, YYB-101, in nude mice bearing human glioblastoma xenografts as a single agent or in combination with temozolomide. HGF neutralization, The extracellular signal-related kinases 1 and 2 (ERK1/2) phosphorylation, and HGF-induced scattering were assessed in HGF-expressing cell lines treated with YYB-101. To support clinical development, we also evaluated the preclinical pharmacokinetics and toxicokinetics in cynomolgus monkeys, and human and cynomolgus monkey tissue was stained with YYB-101 to test tissue cross-reactivity. We found that YYB-101 inhibited cMET activation in vitro and suppressed tumor growth in the orthotopic mouse model of human glioblastoma. Combination treatment with YYB-101 and temozolomide decreased tumor growth and increased overall survival compared with the effects of either agent alone. Five cancer-related genes (TMEM119, FST, RSPO3, ROS1 and NBL1) were overexpressed in YYB-101-treated mice that showed tumor regrowth. In the tissue cross-reactivity assay, critical cross-reactivity was not observed. The terminal elimination half-life was 21.7 days. Taken together, the in vitro and in vivo data demonstrated the anti-tumor efficacy of YYB-101, which appeared to be mediated by blocking the HGF/cMET interaction. The preclinical pharmacokinetics, toxicokinetics and tissue cross-reactivity data support the clinical development of YYB-101 for advanced cancer.

Bone Regeneration with MMP sensitive hyaluronic acid-based hydrogel, rhBMP-2 and nanoparticles in rat calvarial critical size defect(CSD) model

As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase( MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model.Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel- Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.

A Case of Mixed Long Carcinoma Composed of Three Cell Type / 대한내과학회지

Carcinoma of lung is classified by histologic cell type, this is based on predominant major cell population by tissue specimen. But in fact a range of 13% to 63% of lung cancer has been appeared to be heterogeneous cell type at the light microscopic level. After treatment of the mixed lung cancer and/or with time there are reports that cell type is changed. Because it is possible that the tumor were pleomorphic and that the antitumor therapy eliminated the more sensitive cell population and permitted the subsequent emergence of the more resistant cell population. The authors reported here, a case of 32 year old female patient with mixed lung cancer composed of three cell type, and this is the first case in Korea.

A Case of Neonatal Hyperthyroidism

No abstract available.

Clinical and Statistical Observation for Low Birth Weight Infants

We made clinical and statistical observation of low birth weight infants who were delivered at Keimyung University hospital during the 2 years and 4 months period from March, 1979 through July, 1981. The results obtained were as follows: 1) The sex ratio for live birth infants, male to female, was 1.28:1, and low-birth-weight infants was 0.96:1. The incidence of low birth weight infants was 66.5 per 1,000 live births. 2) The etiology of low-birth-weight infants, in order of frequency was: 1st, multiple pregncy, 2nd , permature ruptupe of membrane, 3rd, toxemia, 4th malposition of fotus and 5th , plaoente previa and abruptio placents. 3) The mortality rate for low-birth-weight infants was 123.5/1,000 low-birth-weight infants. 4) Premature percentage of low-birth-weight infants was 61.7%. 5) A sharp decrease in mortality rate was observed when infants weight over 1,750 grams at birth, and over 35 weeks gestation period. Therefore delayed delivery was recommendec. 6) Of all deaths of low-birth-weight infants in the nuracry, 85.9% died within 24 hours of birth, and 82.9% within 48 hours of birth. 7) There was no difference between male and female infants regarding mean helight, mean head circumference and mena chest cincumference according to weight.

Clinical and Statistical Observation for Low Birth Weight Infants

We made clinical and statistical observation of low birth weight infants who were delivered at Keimyung University hospital during the 2 years and 4 months period from March, 1979 through July, 1981. The results obtained were as follows: 1) The sex ratio for live birth infants, male to female, was 1.28:1, and low-birth-weight infants was 0.96:1. The incidence of low birth weight infants was 66.5 per 1,000 live births. 2) The etiology of low-birth-weight infants, in order of frequency was: 1st, multiple pregncy, 2nd , permature ruptupe of membrane, 3rd, toxemia, 4th malposition of fotus and 5th , plaoente previa and abruptio placents. 3) The mortality rate for low-birth-weight infants was 123.5/1,000 low-birth-weight infants. 4) Premature percentage of low-birth-weight infants was 61.7%. 5) A sharp decrease in mortality rate was observed when infants weight over 1,750 grams at birth, and over 35 weeks gestation period. Therefore delayed delivery was recommendec. 6) Of all deaths of low-birth-weight infants in the nuracry, 85.9% died within 24 hours of birth, and 82.9% within 48 hours of birth. 7) There was no difference between male and female infants regarding mean helight, mean head circumference and mena chest cincumference according to weight.

HLA Type in Bronchial Asthma and Atopic Dermatitis

Since the histocompatibility was discovered, we have studied and determined many HLA types till the last workshop held in Los Angeles, USA, 1980. Nowadays we apply HLA types to organ transplantation, transfusion of blood, identification of paternity, study of ethnology and diagnosis of a specific disease. In the present study, 20 cases of bronchial asthma and 15 atopic dermatitis patients were selected. HLA types, serum IgE values and eosinophil count were analized and the data were compared with that of control group. The results were as follows: 1) Eosinophilia appeared in both bronchial asthma and atopic dermatitis 50% and 20% respectively. 2) The P-Values between the mean of serum IgE of bronchial asthma and control and atopic dermatitis and control were P<0.01 and P<0.05 respectively, which were statistically significant. 3) HLA type shows no statitically significant relationship between control group and bronchial asthma and atopic dermatitis.

HLA Type in Bronchial Asthma and Atopic Dermatitis

Since the histocompatibility was discovered, we have studied and determined many HLA types till the last workshop held in Los Angeles, USA, 1980. Nowadays we apply HLA types to organ transplantation, transfusion of blood, identification of paternity, study of ethnology and diagnosis of a specific disease. In the present study, 20 cases of bronchial asthma and 15 atopic dermatitis patients were selected. HLA types, serum IgE values and eosinophil count were analized and the data were compared with that of control group. The results were as follows: 1) Eosinophilia appeared in both bronchial asthma and atopic dermatitis 50% and 20% respectively. 2) The P-Values between the mean of serum IgE of bronchial asthma and control and atopic dermatitis and control were P<0.01 and P<0.05 respectively, which were statistically significant. 3) HLA type shows no statitically significant relationship between control group and bronchial asthma and atopic dermatitis.

A Case of Chylous Ascites

No abstract available.

There cases of Hereditary Anhidrotic Ectodermal Dysplasia

Hereditary anhidrotic ectodermal dysplasia is a rare hereditary condition in which certain structures derived from the ectoderm are undeveloped or underdeveloped, although, on a rarity, mesodermal or endodermal derivatives may be associated. Recently, we enperienced 3-cases of anhidrotic ectodermal dysplasia in 3 months male infant and in brothers aged 4(1/2) months and 22 months. They had abscence of sweating, hypotrichosis and defective dentition, which was characteristic features of this disorder. They seemed to be inherited a sex linked recessive fashion in their family ground. We established the diagnosis with this clinical features, their familial history, and skin biopsy. The first case of these was associated with congenital heart disease, a mesodermal derivative. But, we couldn't explain the associations of this anhidrotic ectodermal dysplasia and the occurance of C.H.D.

A Case of Progressive Muscular Dystrophy

We want to report one case of Duchenne type muscular dystrophy in a female. This patient was seven years of age. Clinical symptoms first appeared at age 3 and progressed insiduously. The Gower's sign was positive. She had a borther who had similar clinical signs and symptoms. He died at age 12. An increased ratio of creatine and creatinine was noted in the patient, with high titers of S.G.O.T., L.D.H. and C.P.K. The muscle biopsy findings showed typical advanced histological changes, that is, great variations in the size of muscle fibers, interstitial fibrosis and infiltration of fat.

A Case of Congenital Choledochal Cyst

We experienced a case of congenital choledochal cyst in a 6 months old korean female infant with complaints of abdominal distension, jaundice, loose stool and a large mass of the abdomenin the right upper quadrant since about 3 months prior to admission. The diagnosis was confirmed by clinical symptoms and signs, physical examination, radiological findings and surgical findings. She was operated with good result and discharged in well condition 28 days after. The review of the related literature was made briefly.

A Case of congenital Tuberculosis

We experienced a case of congenital tuberculosis in 17 days old female infant who had miliary tuberculosis. She was admitted at the gae fo 17 days with a history of high fever and irritavility for 2 days, when she seemed to be weak, underdeveloped and undernourished, and there were diminished aeration on the right lung fields, hpatosplenomegaly,and stat ionary weight She was lst child born of 30 year-old woman after a 6 year of infertility and was normally born at the 37 weeks?gestation period with 2,500 Gm. Weight. She began to cough at the age of 7 days. Her mother, aged 28, was diagnosed as having tuberculous pelvic peritonitis, when she was operated on the left salphingostomy. Infan's chest film showed miliary tubercuous pattern and gastric washing exam. For A.F.B was positive but tuberculin skin test was negative. Finding of endometrial biopsy of mother was consistent with tuberculous eddometritis Diagnosis was established by onset,chilical syndrom,gastric washing, chest film, family history and maternal endometrial biopsy. The reviw of the related literature was made briefly.

A Cases of Autoimmune Hemolytic Anemia

In the autoimmune hemolytic anemia abnormal antibodies directed against red cells are produced by the patient himself. The pathologic mechanism in which erythrocyte survival is decreased as a result of the deposition of specific antibody on the red cell surface is uncertain. The abnormal antibody is most freqeuntly of the lgG or IgM class and occasionally may be IgA. A case of autoimmune hemolytic anemia was confirmed by CBC and Coombs test in a 11years old boy. We are reporting the laboratory data and our experiences of the patient with the reviews of the literatures.

A Case of Disseminated Neuroblastoma

Neuroblastoma is one of the most common malignant tumor in infant and children, which arises from sympathetic nerveous system. this is almost always lethal in children when regional extension or dissemination exists at the time of diagnosis. An exception exists in a special pattern of metastasis to the liver, skin or bone mawow which has batter prognosis. We had experienced a case of generalized disseminated neuroblastoma in 7years and 6 month olds girl which was arised from posterior mediastinum. It was confirmed histologically and by a value of urine VMA. So, we had treated with anticancer drugs I.V. for 68 weeks but the patient expired on May, 1978. Pertinent literatures concerning neuroblastoma were also reviewed.